Siglec-7 antibody | S7.7
Mouse anti Human Siglec-7:FITC
- Product Type
- Monoclonal Antibody
- Clone
- S7.7
- Isotype
- IgG1
- Specificity
- Siglec-7
Antibody Quality and Performance Guarantee
| Mouse anti Human Siglec-7 antibody, cloneS7.7 recognizes human Sialic acid-binding Ig-like lectin 7, otherwise known as CD328, a putative adhesion molecule and member of the Ig superfamily, predominantly expressed by both resting and activated natural killer (NK) cells, and also at lower levels by monocytes, granulocytes, monocyte-derived macrophages and dendritic cells, and a small population of CD8+ memory T cells. Classed as a CD33-related Siglec, Siglec-7 preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid, and acts as a novel inhibitory receptor for NK cells, mediating the inhibition of NK cell cytotoxicity, and implicated in the regulation of NK cell and T cell activation, and hematopoiesis. Mouse anti Human Siglec-7 antibody, cloneS7.7 is a blocking antibody (Avril et al. 2006), and has been shown to recognize Siglec-7 in Rhesus monkey (Jaroenpool et al. 2007). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Siglec-7 3T3 transfected cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice, were fused with cells of the Sp2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
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| Description | Human Seroblock | ||||||
References for Siglec-7 antibody
-
Nicoll, G. et al. (1999) Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes.
J Biol Chem. 274 (48): 34089-95. -
Nguyen, D.H. et al. (2006) Loss of Siglec expression on T lymphocytes during human evolution.
Proc Natl Acad Sci U S A. 103 (20): 7765-70. -
Avril T et al. (2006) Sialic acid-binding immunoglobulin-like lectin 7 mediates selective recognition of sialylated glycans expressed on Campylobacter jejuni lipooligosaccharides.
Infect Immun. 74 (7): 4133-41. -
Jaroenpool, J. et al. (2007) Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates.
Cell Immunol. 250 (1-2): 91-104.
- Synonyms
- CD328
- UniProt
- Q9Y286
- Entrez Gene
- SIGLEC7
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
MCA5782F
0112
0112R
162821
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