SHC1 p66/p52/p46 antibody | F02/2H5

Mouse anti SHC1

Product Type
PrecisionAb Monoclonal
Clone
F02/2H5
Isotype
IgG1
Format
Purified
Specificity
SHC1 p66/p52/p46

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MCA6170
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Mouse anti SHC1 p66/p52/p46 antibody recognizes SHC-transforming protein 1, also known as SHC and SHCA.

SHC1 is a uniquitously-expressed signaling adapter, linking activated growth factor receptors to signaling pathways. Importantly, EGFR signaling is mediated by phosphorylation and interactions of SHC1 protein, defining output from the EGFR pathway (Zheng et al. 2013). SHC1 exists as three functionally-distinct isoforms with molecular weights 46 kDa, 52 kDa and 66 kDa. These isoforms are encoded from the same gene, sharing a common structure and each possessing several phosphorylation sites (Mehlitz et al. 2010). SHC1 plays an oncogenic role in several cancer types (An et al. 2016). Isoforms of SHC1 may have distinct roles in disease. Notably, the p52 isoform of SHC1 contributes to breast cancer initiation (Wright et al. 2019).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti SHC1 antibody detects bands of approximately 51 kDa, 55 kDa, and 66 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant protein, aa 484-583 of human SHC1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P29353
Entrez Gene
SHC1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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