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SERPINH1 antibody | AB01/3C1

Mouse anti SERPINH1

Product Type
PrecisionAb Monoclonal

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti SERPINH1 antibody recognizes serpin H1, also known as HSP47, AsTP3 and colligin. SERPINH1 is a member of the serpin superfamily of protease inhibitors, critical for control of many proteolytic cascades including the coagulation cascade in mammals (Law et al. 2006). SERPINH1 is a key regulator of collagen synthesis. It is essential for appropriate folding of pro-collagen in the endoplasmic reticulum, and by association is involved with embryonic development (Kumar et al. 2017). SERPINH1 and has been implicated in collagen-related diseases including brittle bone disease, keloid scars and lung fibrosis (Tian et al. 2020). SERPINH1 is highly expressed in a range of cancers including pancreatic cancer, glioma, and clear cell renal cell carcinoma, and can drive malignant behaviour of cancer cells (Qi et al. 2018). PrecisionAb Antibodies

Target Species
Western Blotting
Mouse anti SERPINH1 detects a band of approximately 44 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
E. coli-derived recombinant protein of amino acids 19-418 of human SERPINH1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
For research purposes only
12 months from date of despatch
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

Entrez Gene
GO Terms
GO:0006986 response to unfolded protein
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005518 collagen binding
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment



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