Sdhb antibody | OTI2A3
Mouse anti Human SDHB antibody recognizes SDHB, also known as iron-sulfur subunit of complex II.
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to Coenzyme Q. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in SDHB gene result in development of paragangliomas (PGL4), pheochromocytoma (PCC) or paraganglioma and gastric stromal sarcoma (PGGSS) suggesting a link between mitochondrial dysfunction and tumorigenesis. (provided by RefSeq, Jul 2008).
Mouse anti Human SDHB antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to Coenzyme Q. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in SDHB gene result in development of paragangliomas (PGL4), pheochromocytoma (PCC) or paraganglioma and gastric stromal sarcoma (PGGSS) suggesting a link between mitochondrial dysfunction and tumorigenesis. (provided by RefSeq, Jul 2008).
Mouse anti Human SDHB antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti SDHB detects a band of approximately 32 kDa in HepG2 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length human recombinant protein of human SDHB produced in E. Coli
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21912
- Entrez Gene
- SDHB
- GO Terms
- GO:0009055 electron carrier activity
- GO:0005515 protein binding
- GO:0046872 metal ion binding
- GO:0005749 mitochondrial respiratory chain complex II
- GO:0006099 tricarboxylic acid cycle
- GO:0022904 respiratory electron transport chain
- GO:0006810 transport
- GO:0008177 succinate dehydrogenase (ubiquinone) activity
- GO:0048039 ubiquinone binding
- GO:0051537 2 iron, 2 sulfur cluster binding
- GO:0051538 3 iron, 4 sulfur cluster binding
- GO:0051539 4 iron, 4 sulfur cluster binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Sdhb antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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