Sdhb antibody | 2A3

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Mouse anti Sdhb

<strong>Mouse anti-human SDHB antibody</strong> recognizes succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, also known as iron-sulfur subunit of complex II. This antibody has been extensively validated for western blotting using whole cell lysates.

Sdhb Antibody with Control Lysate

<strong>Mouse anti-human SDHB antibody</strong> recognizes succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, also known as iron-sulfur subunit of complex II. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
2A3
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00457 WB 100 µl
VMA00457KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human SDHB antibody recognizes SDHB, also known as iron-sulfur subunit of complex II.

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to Coenzyme Q. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in SDHB gene result in development of paragangliomas (PGL4), pheochromocytoma (PCC) or paraganglioma and gastric stromal sarcoma (PGGSS) suggesting a link between mitochondrial dysfunction and tumorigenesis. (provided by RefSeq, Jul 2008).

Mouse anti Human SDHB antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length human recombinant protein of human SDHB produced in E. Coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P21912 Related reagents
Entrez Gene
SDHB Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005515 protein binding
GO:0046872 metal ion binding
GO:0005749 mitochondrial respiratory chain complex II
GO:0006099 tricarboxylic acid cycle
GO:0022904 respiratory electron transport chain
GO:0006810 transport
GO:0008177 succinate dehydrogenase (ubiquinone) activity
GO:0048039 ubiquinone binding
GO:0051537 2 iron, 2 sulfur cluster binding
GO:0051538 3 iron, 4 sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Sdhb antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti SDHB detects a band of approximately 32 kDa in HepG2 cell lysate
Western Blotting
Anti SDHB detects a band of approximately 32 kDa in HepG2 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB