S100A9 antibody | OTI10D5

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Anti S100A9 Antibody, clone OTI10D5 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with S100A9 antibody (**VMA00199**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 17 second exposure. Arrow points to S100A9 (molecular weight 13 kDa).

Mouse anti S100A9

S100A9 antibody recognizes the protein S100A9, also known as MRP-14, S100 calcium-binding protein A9 (calgranulin B), calgranulin B, calprotectin L1H subunit, leukocyte L1 complex heavy chain or migration inhibitory factor-related protein 14.

S100A9 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI10D5
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00199	WB	100 µl
VMA00199KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human S100A9 antibody recognizes S100A9, also known as MRP-14, S100 calcium-binding protein A9 (calgranulin B), calgranulin B, calgranulin-B, calprotectin L1H subunit, leukocyte L1 complex heavy chain and migration inhibitory factor-related protein 14.

The protein encoded by the S100A9 gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis (provided by RefSeq, Jul 2008).

Mouse anti Human S100A9 antibody detects a band of 13 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti S100A9 detects a band of approximately 13 kDa in MCF7 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human S100A9 (NP_002956) produced in SF9 cells
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P06702 Related reagents
Entrez Gene: S100A9 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0005509 calcium ion binding; GO:0005576 extracellular region; GO:0004871 signal transducer activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005856 cytoskeleton; GO:0006935 chemotaxis; GO:0006954 inflammatory response; GO:0007267 cell-cell signaling
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of S100A9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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