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S100A8 antibody

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Anti S100A8 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with S100A8 antibody (VMA00595) followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to S100A8 (molecular weight 11 kDa).

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Mouse anti S100A8

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
S100A8

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Product Code Applications Pack Size List Price Your Price Qty
VMA00595
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WB 100 µl loader
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Mouse anti Human S100A8 antibody recognizes S100A8, also known as MRP-8, S100 calcium-binding protein A8 (calgranulin A), calprotectin L1L subunit, leukocyte L1 complex light chain or migration inhibitory factor-related protein 8.

The protein encoded by S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis (provided by RefSeq, Jul 2008).

Mouse anti Human S100A8 antibody detects a band of 11 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti S100A8 antibody detects a band of approximately 11 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody affinity purified on immunogen from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Full length recombinant human S100A8
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P05109
Entrez Gene
S100A8
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0006935 chemotaxis
GO:0006954 inflammatory response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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