S100 antibody | 8B10
No associated image currently available
Mouse anti Human S100 Protein
- Product Type
- Monoclonal Antibody
- Clone
- 8B10
- Isotype
- IgG1
- Specificity
- S100
Antibody Quality and Performance Guarantee
| Mouse anti Human S100 Protein antibody, clone 8B10 recognizes S100 protein derived from human brain tissue. It is a 21kDa acidic calcium-binding protein synthesised by astroglial cells. It can be found predominantly as two isoforms, the alpha-beta heterodimer (S100a) and the beta-beta homodimer (S100b). The S100 family of proteins have been implicated in a large variety of intracellular and extracellular regulatory activities, such as cellular energy metabolism, cytoskeleton modification, cell proliferation and differentiation. When glial cells are damaged, S100 protein is leaked into the extracellular matrix and cerebrospinal fluid, further releasing into the bloodstream. S100 protein can be used as a sensitive and reliable marker for nervous system damage, such as after brain damage or acute stroke. Mouse anti Human S100 Protein antibody, clone 8B10 recognizes both S100a (alpha-beta) and S100b (beta-beta). Mouse anti Human S100 Protein antibody, clone 8B10 is sensitive to EDTA and other bivalent-binding agents. Improved performance can be obtained in the presence of 5mM CaCl2 in assay buffers. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human brain S100 protein
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for S100 antibody
-
Valencia, K. et al. (2012) Inhibition of collagen receptor discoidin domain receptor-1 (DDR1) reduces cell survival, homing, and colonization in lung cancer bone metastasis.
Clin Cancer Res. 18 (4): 969-80. -
Etherington S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
Journal of Zoology. 300 (1): 27-35.
- RRID
- AB_1102841
MCA2769
180705
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with S100 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up