S100 antibody | 6G1
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Mouse anti Human S100 Protein
- Product Type
- Monoclonal Antibody
- Clone
- 6G1
- Isotype
- IgG1
- Specificity
- S100
Antibody Quality and 
Unrivaled 
| Mouse anti Human S100 Protein antibody, clone 6G1 recognizes S100 protein derived from human brain tissue. It is a ~21 kDa acidic calcium-binding protein synthesised by astroglial cells. It can be found predominantly as two isoforms, the alpha-beta heterodimer (S100a) and the beta-beta homodimer (S100b). The S100 family of proteins have been implicated in a large variety of intracellular and extracellular regulatory activities, such as cellular energy metabolism, cytoskeleton modification, cell proliferation and differentiation. When glial cells are damaged, S100 protein is leaked into the extracellular matrix and cerebrospinal fluid, further releasing into the bloodstream. As such, S100 protein can be used as a sensitive and reliable marker for nervous system damage, such as after brain damage or acute stroke. Mouse anti Human S100 Protein antibody, clone 6G1 recognizes both S100a (alpha-beta) and S100b (beta-beta). Mouse anti Human S100 Protein antibody, clone 6G1 is sensitive to EDTA and other bivalent-binding agents. Better performance can be obtained in the presence of 5mM CaCl2 in assay buffers. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human brain S100 protein.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for S100 antibody
-
Paus, E. et al. (2011) TD-11 workshop report: characterization of monoclonal antibodies to S100 proteins.
Tumour Biol. 32 (1): 1-12. -
Wang, Q. et al. (2012) Evaluation of human brain damage in fatalities due to extreme environmental temperature by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP), S100β and single-stranded DNA (ssDNA) immunoreactivities.
Forensic Sci Int. 219 (1-3): 259-64.
- RRID
- AB_1102842
View more products with S100 specificity
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