RRM2 antibody | 1E1

Mouse anti Human RRM2:Preservative Free

Product Type
Monoclonal Antibody
Clone
1E1
Isotype
IgG1
Specificity
RRM2

Product Code Applications Pack Size List Price Your Price Qty
MCA3434Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human RRM2 antibody, clone 1E1 recognizes human Ribonucleoside-diphosphate reductase subunit M2, also known as RRM2 or Ribonucleotide reductase small subunit. RRM2 is a 389 amino acid ~ 45 kDa intracellular antigen involved in the synthesis of precursors required for DNA synthesis (UniProt: P31350).

Mouse anti Human RRM2 antibody, clone 1E1 has been used for the detection of RRM2 in human bladder cancer cell lines by western blotting (Kerr et al. 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-111 of human RRM2.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for RRM2 antibody

  1. Tang, L. Y. et al. (2007) Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction.
    Mol Cell Proteomics. 6: 1952-67.
  2. Kerr, M. et al. (2014) Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer.
    Clin Cancer Res. 20 (21): 5435-45.

RRID
AB_2180397
UniProt
P31350
Entrez Gene
RRM2
GO Terms
GO:0006260 DNA replication
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0005829 cytosol
GO:0004748 ribonucleoside-diphosphate reductase activity
GO:0055114 oxidation-reduction process
GO:0009186 deoxyribonucleoside diphosphate metabolic process
GO:0009263 deoxyribonucleotide biosynthetic process
GO:0015949 nucleobase, nucleoside and nucleotide interconversion
GO:0046914 transition metal ion binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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