RPS6KB2 antibody

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Mouse anti RPS6KB2

Mouse anti human RPS6KB2 antibody recognizes ribosomal protein S6 kinase beta-2, also known as p70 S6 kinase beta, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, or serine/threonine-protein kinase 14B. This antibody has been extensively validated for western blotting using whole cell lysates.

RPS6KB2 Antibody with Control Lysate

Mouse anti human RPS6KB2 antibody recognizes ribosomal protein S6 kinase beta-2, also known as p70 S6 kinase beta, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, S6 kinase-related kinase, S6K-beta, S6K-beta-2, p70 S6K-beta, p70 S6KB or serine/threonine-protein kinase 14B.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00358 WB 100 µl
VMA00358KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human RPS6KB2 antibody recognizes ribosomal protein S6 kinase beta-2, also known as P70 S6 kinase beta, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, S6 kinase-related kinase, S6K-beta, S6K-beta-2, p70 S6K-beta, p70 ribosomal S6 kinase beta, p70-S6K 2, serine/threonine-protein kinase 14 beta and serine/threonine-protein kinase 14B.

The RPS6KB2 gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation (provided by RefSeq, Jul 2008).

Mouse anti Human RPS6KB2 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RPS6KB2 detects a band of approximately 70 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant RPS6KB2 protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee
As supplied, 12 months from date of despatch.
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q9UBS0 Related reagents
Entrez Gene
RPS6KB2 Related reagents
GO Terms
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006412 translation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0045948 positive regulation of translational initiation
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPS6KB2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
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