RPS6KB2 antibody

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Mouse anti RPS6KB2

Mouse anti human RPS6KB2 antibody recognizes ribosomal protein S6 kinase beta-2, also known as p70 S6 kinase beta, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, or serine/threonine-protein kinase 14B. This antibody has been extensively validated for western blotting using whole cell lysates.

RPS6KB2 Antibody with Control Lysate

Mouse anti human RPS6KB2 antibody recognizes ribosomal protein S6 kinase beta-2, also known as p70 S6 kinase beta, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, S6 kinase-related kinase, S6K-beta, S6K-beta-2, p70 S6K-beta, p70 S6KB or serine/threonine-protein kinase 14B.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00358	WB	100 µl
VMA00358KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human RPS6KB2 antibody recognizes ribosomal protein S6 kinase beta-2, also known as P70 S6 kinase beta, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, S6 kinase-related kinase, S6K-beta, S6K-beta-2, p70 S6K-beta, p70 ribosomal S6 kinase beta, p70-S6K 2, serine/threonine-protein kinase 14 beta and serine/threonine-protein kinase 14B.

The RPS6KB2 gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation (provided by RefSeq, Jul 2008).

Mouse anti Human RPS6KB2 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti RPS6KB2 detects a band of approximately 70 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Recombinant RPS6KB2 protein

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee: As supplied, 12 months from date of despatch.
Guarantee: As supplied, 12 months from date of despatch

More Information

UniProt: Q9UBS0 Related reagents
Entrez Gene: RPS6KB2 Related reagents
GO Terms: GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0006412 translation; GO:0048015 phosphatidylinositol-mediated signaling; GO:0045948 positive regulation of translational initiation; GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of RPS6KB2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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