RPS6 antibody

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Anti RPS6 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RPS6 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to RPS6 (molecular weight 32 kDa)

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Mouse anti RPS6

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: RPS6

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VMA00304 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human RPS6 antibody recognizes the 40S ribosomal protein S6, also known as 40S ribosomal protein S6, phosphoprotein NP33.

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. Encoded by the RPS6 gene, 40S ribosomal protein S6 is a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPS6 dispersed through the genome (provided by RefSeq, Jul 2008).

Mouse anti Human RPS6 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti RPS6 detects a band of approximately 32 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: RPS6 recombinant protein
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P62753
Entrez Gene: RPS6
GO Terms: GO:0022627 cytosolic small ribosomal subunit; GO:0005515 protein binding; GO:0003735 structural constituent of ribosome; GO:0005730 nucleolus; GO:0006364 rRNA processing; GO:0006414 translational elongation; GO:0006415 translational termination; GO:0008286 insulin receptor signaling pathway; GO:0019083 viral transcription; View More GO Terms; GO:0031018 endocrine pancreas development; GO:0031929 TOR signaling cascade; GO:0042274 ribosomal small subunit biogenesis; GO:0042593 glucose homeostasis; GO:0043065 positive regulation of apoptosis