RPL10 antibody | OTI6B11
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WB ResetMouse anti RPL10
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI6B11
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- RPL10
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| Mouse anti Human RPL10 antibody, clone OTI6B11 recognizes RPL10, also known as Wilms tumor-related protein, laminin receptor homolog and tumor suppressor QM. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. The RPL10 gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro. the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. Alternative splicing results in multiple transcript variants. RPL10 also uses multiple polyA signals, with the 3'-most polyA signal overlapping the deoxyribonuclease I-like 1 gene on the opposite strand. RPL10 is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL10 dispersed through the genome (provided by RefSeq, Feb 2012). Mouse anti Human RPL10 antibody, clone OTI6B11 detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti RPL10 detects a band of approximately 25 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human RPL10 (NP_006004) produced in E.coli
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P27635
- Entrez Gene
- RPL10
- GO Terms
- GO:0022625 cytosolic large ribosomal subunit
- GO:0003735 structural constituent of ribosome
- GO:0005783 endoplasmic reticulum
- GO:0006414 translational elongation
- GO:0006415 translational termination
- GO:0019083 viral transcription
- GO:0031018 endocrine pancreas development
View more products with RPL10 specificity
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