RPA2 antibody | OTI9A1

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Mouse anti RPA2

Mouse anti human RPA2 antibody recognizes replication protein A 32 kDa subunit, also known as RPA32, RF-A protein 2 or replication factor A protein 2. This antibody has been extensively validated for western blotting using whole cell lysates.

RPA2 Antibody with Control Lysate

Mouse anti human RPA2 antibody recognizes replication protein A 32 kDa subunit, also known as RPA32, RF-A protein 2 or replication factor A protein 2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI9A1
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00366 WB 100 µl
VMA00366KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human RPA2 antibody recognizes replication protein A 32 kDa subunit, also known as RPA2 and RF-A protein 2.

Mouse anti Human RPA2 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RPA2 detects a band of approximately 32 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human RPA2 (NP_002937) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P15927 Related reagents
Entrez Gene
RPA2 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0000722 telomere maintenance via recombination
GO:0000730 DNA recombinase assembly
GO:0003697 single-stranded DNA binding
GO:0005662 DNA replication factor A complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0016605 PML body
GO:0032201 telomere maintenance via semi-conservative replication
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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