RPA1 antibody
Mouse anti RPA1
RPA1 antibody recognizes replication protein A 70 kDa DNA-binding subunit, also known as MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1 or single-stranded DNA-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.
RPA1 Antibody with Control Lysate
RPA1 antibody recognizes replication protein A 70 kDa DNA-binding subunit, also known as MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1 or single-stranded DNA-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00158 | WB | 100 µl | ||||
| VMA00158KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human RPA1 antibody recognizes RPA1, also known as MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit and single-stranded DNA-binding protein.
Mouse anti Human RPA1 antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human RPA1 antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RPA1 detects a band of approximately 68 kDa in HEK293 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified recombinant fragment of human RPA1 expressed in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P27694 Related reagents
- Entrez Gene
- RPA1 Related reagents
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0000718 nucleotide-excision repair, DNA damage removal
- GO:0000722 telomere maintenance via recombination
- GO:0000730 DNA recombinase assembly
- GO:0003697 single-stranded DNA binding
- GO:0046872 metal ion binding
- GO:0005737 cytoplasm
- GO:0005662 DNA replication factor A complex
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006297 nucleotide-excision repair, DNA gap filling
- GO:0015629 actin cytoskeleton
- GO:0016605 PML body
- GO:0032201 telomere maintenance via semi-conservative replication
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RPA1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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