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RPA1 antibody

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Western Blot Validated
Anti RPA1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with RPA1 antibody (VMA00158) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 200 second exposure. Arrow points to RPA1 (molecular weight 68 kDa).

Mouse anti RPA1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00158
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human RPA1 antibody recognizes RPA1, also known as MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit and single-stranded DNA-binding protein.

Mouse anti Human RPA1 antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RPA1 detects a band of approximately 68 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human RPA1 expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P27694 Related reagents
Entrez Gene
RPA1 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0000722 telomere maintenance via recombination
GO:0000730 DNA recombinase assembly
GO:0003697 single-stranded DNA binding
GO:0046872 metal ion binding
GO:0005737 cytoplasm
GO:0005662 DNA replication factor A complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0015629 actin cytoskeleton
GO:0016605 PML body
GO:0032201 telomere maintenance via semi-conservative replication
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RPA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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Request a different product with the RPA1 specificity