ROCK1 antibody | AB01/1F3

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Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ROCK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to ROCK1 (molecular weight 185 kDa).

Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of ROCK1 from HepG2 whole cell lysate. The Input lane is 10% input; the ROCK1-IP lane is the immunoprecipitation with the ROCK1 antibody (2.5 µg, **VMA00614**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using ROCK1 antibody (1/1000, **VMA00614**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, 1705047) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to ROCK1.

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WB IP

Mouse anti ROCK1

Product Type: PrecisionAb Monoclonal
Clone: AB01/1F3
Isotype: IgG1
Format: Purified
Specificity: ROCK1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00614 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human ROCK1 antibody recognizes ROCK1, also known as P160 ROCK or rho-associated protein kinase 1.

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18 (provided by RefSeq, Aug 2015).

Mouse anti Human ROCK1 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti ROCK1 antibody detects a band of approximately 185 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human ROCK1 (aa 629-829)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q13464
Entrez Gene: ROCK1
GO Terms: GO:0000139 Golgi membrane; GO:0000910 cytokinesis; GO:0030036 actin cytoskeleton organization; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005814 centriole; GO:0006468 protein phosphorylation; View More GO Terms; GO:0006921 cellular component disassembly involved in apoptosis; GO:0007266 Rho protein signal transduction; GO:0007411 axon guidance; GO:0042802 identical protein binding; GO:0022614 membrane to membrane docking; GO:0050901 leukocyte tethering or rolling