ROCK1 antibody | AB01/1F3
Mouse anti Human ROCK1 antibody recognizes ROCK1, also known as P160 ROCK or rho-associated protein kinase 1.
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18 (provided by RefSeq, Aug 2015).
Mouse anti Human ROCK1 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18 (provided by RefSeq, Aug 2015).
Mouse anti Human ROCK1 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti ROCK1 antibody detects a band of approximately 185 kDa in HepG2 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human ROCK1 (aa 629-829)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q13464 Related reagents
- Entrez Gene
- ROCK1 Related reagents
- GO Terms
- GO:0000139 Golgi membrane
- GO:0000910 cytokinesis
- GO:0030036 actin cytoskeleton organization
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005814 centriole
- GO:0006468 protein phosphorylation
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007266 Rho protein signal transduction
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0022614 membrane to membrane docking
- GO:0050901 leukocyte tethering or rolling
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ROCK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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