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ROCK1 antibody | AB01/1F3

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Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ROCK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to ROCK1 (molecular weight 185 kDa).
Anti ROCK1 Antibody, clone AB01/1F3 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of ROCK1 from HepG2 whole cell lysate. The Input lane is 10% input; the ROCK1-IP lane is the immunoprecipitation with the ROCK1 antibody (2.5 µg, VMA00614); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using ROCK1 antibody (1/1000, VMA00614) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, 1705047) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to ROCK1.

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WB IP

Mouse anti ROCK1

Product Type
PrecisionAb Monoclonal
Clone
AB01/1F3
Isotype
IgG1
Format
Purified
Specificity
ROCK1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00614
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IP WB 100 µl loader
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Mouse anti Human ROCK1 antibody recognizes ROCK1, also known as P160 ROCK or rho-associated protein kinase 1.

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18 (provided by RefSeq, Aug 2015).

Mouse anti Human ROCK1 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti ROCK1 antibody detects a band of approximately 185 kDa in HepG2 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human ROCK1 (aa 629-829)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13464
Entrez Gene
ROCK1
GO Terms
GO:0000139 Golgi membrane
GO:0000910 cytokinesis
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005814 centriole
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007266 Rho protein signal transduction
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0022614 membrane to membrane docking
GO:0050901 leukocyte tethering or rolling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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