RNF20 antibody | AB06/4F9

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Mouse anti RNF20

Product Type
PrecisionAb Monoclonal
Clone
AB06/4F9
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00968
Datasheet
IP WB
SDS
100 µl loader

Mouse anti RNF20 antibody recognizes E3 ubiquitin-protein ligase BRE1A, also known as BRE1A. RNF20 is a RING domain E3 ubiquitin ligase and mediates ubiquitin transfer to target proteins. RNF20 plays many roles in transcription, cell division, heat shock responses, and adipogenesis (Jeon et al. 2020). In development, RNF20 is essential for plasticity of embryonic stem cells and controls astrocyte differentiation during brain development (Liang et al. 2018). Alterations in the gene encoding RNF20 have been identified in a variety of human cancers including breast, lung and prostate cancer, clear cell renal cell carcinoma and mixed lineage leukemia. Reduced expression of RNF20 appears to be linked to genome instability, and RNF20 appears to act as a tumor suppressor in cancer types driven by chronic inflammation (Sethi et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti RNF20 detects a band of approximately 120 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-526 of human RNF20
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q5VTR2
Entrez Gene
RNF20
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RNF20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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