RNF20 antibody | AB06/4F9

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Anti RNF20 Antibody, clone AB06/4F9 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RNF20 Antibody, clone AB06/4F9 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti RNF20 Antibody, clone AB06/4F9 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RNF20 antibody (1/2000, **VMA00968**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to RNF20 (molecular weight 120 kDa)

Anti RNF20 Antibody, clone AB06/4F9 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of RNF20 from HEK293 whole cell lysate. The Input lane is 10% input; the RNF20-IP lane is the immunoprecipitation with the RNF20 antibody (2.5 µg, **VMA00986**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA929). Western blot analysis was performed using RNF20 antibody (1/1000, **VMA00986**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, 1705047) and visualized on the ChemiDoc MP with 36.5 second exposure. Arrow points to RNF20.

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Mouse anti RNF20

Product Type: PrecisionAb Monoclonal
Clone: AB06/4F9
Isotype: IgG2a
Format: Purified
Specificity: RNF20

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VMA00968 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti RNF20 antibody recognizes E3 ubiquitin-protein ligase BRE1A, also known as BRE1A. RNF20 is a RING domain E3 ubiquitin ligase and mediates ubiquitin transfer to target proteins. RNF20 plays many roles in transcription, cell division, heat shock responses, and adipogenesis (Jeon et al. 2020). In development, RNF20 is essential for plasticity of embryonic stem cells and controls astrocyte differentiation during brain development (Liang et al. 2018). Alterations in the gene encoding RNF20 have been identified in a variety of human cancers including breast, lung and prostate cancer, clear cell renal cell carcinoma and mixed lineage leukemia. Reduced expression of RNF20 appears to be linked to genome instability, and RNF20 appears to act as a tumor suppressor in cancer types driven by chronic inflammation (Sethi et al. 2018).

Target Species: Human
Western Blotting: Mouse anti RNF20 detects a band of approximately 120 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-526 of human RNF20
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.