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RNF20 antibody | AB06/4F9

Mouse anti RNF20

Product Type
PrecisionAb Monoclonal

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti RNF20 antibody recognizes E3 ubiquitin-protein ligase BRE1A, also known as BRE1A. RNF20 is a RING domain E3 ubiquitin ligase and mediates ubiquitin transfer to target proteins. RNF20 plays many roles in transcription, cell division, heat shock responses, and adipogenesis (Jeon et al. 2020). In development, RNF20 is essential for plasticity of embryonic stem cells and controls astrocyte differentiation during brain development (Liang et al. 2018). Alterations in the gene encoding RNF20 have been identified in a variety of human cancers including breast, lung and prostate cancer, clear cell renal cell carcinoma and mixed lineage leukemia. Reduced expression of RNF20 appears to be linked to genome instability, and RNF20 appears to act as a tumor suppressor in cancer types driven by chronic inflammation (Sethi et al. 2018). PrecisionAb Antibodies

Target Species
Western Blotting
Mouse anti RNF20 detects a band of approximately 120 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
E. coli-derived recombinant protein of amino acids 1-526 of human RNF20
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
For research purposes only
12 months from date of despatch
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Entrez Gene



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