RNA Pol II CTD (pSer5) antibody | AbD37525

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Anti RNA Pol II CTD (pSer5) Antibody, clone AbD37525 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RNA Pol II CTD (pSer5) Antibody, clone AbD37525 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of HeLa cell lysate probed with Human anti RNA Pol II CTD (pSer5) antibody (1/1000, **VMA00909**) followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 antibody (1/2500, STAR126P). Membranes were treated with (+) or without (-) lambda phosphatase as indicated and visualized on the ChemiDoc MP Imaging System. Arrow points to RNA Pol II CTD (pSer5).

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Human anti RNA Pol II CTD (pSer5)

Product Type: PrecisionAb Monoclonal
Clone: AbD37525
Isotype: HuCAL Fab bivalent
Format: Purified
Specificity: RNA Pol II CTD (pSer5)

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Human anti RNA Pol II CTD (pSer5) antibody is a recombinant Fab antibody fragment in the format Fab-Max-FH with specificity for DNA-directed RNA polymerase II subunit RPB1, otherwise known as RNAP II, RNA Pol II and Pol II, when phosphorylated at serine 5. Pol II forms part of a highly-conserved initiator complex for transcription of gene transcription, directing RNA synthesis with the help of general initiation factors (Sainsbury et al. 2015). In fact, Pol II is able to transcribe all protein-coding genes and most non-coding RNA genes in eukaryotes, providing an essential role (Kuehner et al. 2011). Pol II undergoes dynamic post-translational modification at its carboxy-terminal domain (CTD) in order to regulate transcription (Harlen and Churchman 2017). The CTD contains the residues Ser2, Ser 5 and Ser 7 within multiple heptad repeats. Phosphorylation at these targets regulates the activities of the CTD, which include coupling transcription and processing of nascent RNA, and transcription elongation and termination (Hsin and Manley 2012).

Target Species: Human
Western Blotting: Human anti RNA Pol II CTD (pSer5) detects a band of approximately 299 kDa in Hela cell lysates
Product Form: A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of an inactive variant of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation: Prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Phospho specific-peptide corresponding to residues surrounding serine 5 of human RPB1 and conjugated to BSA and human transferrin
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

His-tag is a registered trademark of EMD Biosciences

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Technical Advice: Technical advice - Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Synonyms: RPB1
UniProt: P24928
Entrez Gene: POLR2A
GO Terms: GO:0000398 nuclear mRNA splicing, via spliceosome; GO:0003677 DNA binding; GO:0003899 DNA-directed RNA polymerase activity; GO:0003968 RNA-directed RNA polymerase activity; GO:0046872 metal ion binding; GO:0005665 DNA-directed RNA polymerase II, core complex; GO:0006283 transcription-coupled nucleotide-excision repair; GO:0006355 regulation of transcription, DNA-dependent; GO:0006367 transcription initiation from RNA polymerase II promoter; View More GO Terms; GO:0006368 transcription elongation from RNA polymerase II promoter; GO:0006370 mRNA capping; GO:0016032 viral reproduction; GO:0031625 ubiquitin protein ligase binding; GO:0050434 positive regulation of viral transcription