RNA Pol II CTD (pSer5) antibody | AbD37525

Human anti RNA Pol II CTD (pSer5)

Product Type
PrecisionAb Monoclonal
Clone
AbD37525
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
RNA Pol II CTD (pSer5)

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Human anti RNA Pol II CTD (pSer5) antibody is a recombinant Fab antibody fragment in the format Fab-Max-FH with specificity for DNA-directed RNA polymerase II subunit RPB1, otherwise known as RNAP II, RNA Pol II and Pol II, when phosphorylated at serine 5. Pol II forms part of a highly-conserved initiator complex for transcription of gene transcription, directing RNA synthesis with the help of general initiation factors (Sainsbury et al. 2015). In fact, Pol II is able to transcribe all protein-coding genes and most non-coding RNA genes in eukaryotes, providing an essential role (Kuehner et al. 2011). Pol II undergoes dynamic post-translational modification at its carboxy-terminal domain (CTD) in order to regulate transcription (Harlen and Churchman 2017). The CTD contains the residues Ser2, Ser 5 and Ser 7 within multiple heptad repeats. Phosphorylation at these targets regulates the activities of the CTD, which include coupling transcription and processing of nascent RNA, and transcription elongation and termination (Hsin and Manley 2012). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Human anti RNA Pol II CTD (pSer5) detects a band of approximately 299 kDa in Hela cell lysates
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of an inactive variant of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 5 of human RPB1 and conjugated to BSA and human transferrin
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

His-tag is a registered trademark of EMD Biosciences

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Technical Advice
Technical advice - Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Synonyms
RPB1
UniProt
P24928
Entrez Gene
POLR2A
GO Terms
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003899 DNA-directed RNA polymerase activity
GO:0003968 RNA-directed RNA polymerase activity
GO:0046872 metal ion binding
GO:0005665 DNA-directed RNA polymerase II, core complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006355 regulation of transcription, DNA-dependent
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0016032 viral reproduction
GO:0031625 ubiquitin protein ligase binding
GO:0050434 positive regulation of viral transcription