RIPK1 antibody
Mouse anti RIPK1
Mouse anti human RIPK1 antibody recognizes receptor-interacting serine/threonine-protein kinase 1, also known as RIP-1, cell death protein RIP or serine/threonine-protein kinase RIP. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00422 | WB | 100 µl |
Mouse anti Human RIPK1 antibody recognizes the RIPK1, also known as RIP-1, cell death protein RIP, receptor interacting protein, receptor-interacting protein 1, receptor-interacting serine/threonine-protein kinase 1 or serine/threonine-protein kinase RIP.
RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RIPK1 detects a band of approximately 78 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E.coli-derived recombinant protein corresponding to aa 1-671 of human RIPK1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q13546 Related reagents
- Entrez Gene
- RIPK1 Related reagents
- GO Terms
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005123 death receptor binding
- GO:0005524 ATP binding
- GO:0006919 activation of caspase activity
- GO:0007257 activation of JUN kinase activity
- GO:0008063 Toll signaling pathway
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0010008 endosome membrane
- GO:0031264 death-inducing signaling complex
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0034612 response to tumor necrosis factor
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043235 receptor complex
- GO:0045087 innate immune response
- GO:0045768 positive regulation of anti-apoptosis
- GO:0046777 protein autophosphorylation
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0060555 induction of necroptosis by extracellular signals
- GO:0070513 death domain binding
- GO:0070926 regulation of ATP:ADP antiporter activity
- GO:0080010 regulation of oxygen and reactive oxygen species metabolic process
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RIPK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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