RIPK1 antibody

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Anti RIPK1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RIPK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 11 second exposure. Arrow points to RIPK1 (molecular weight 78 kDa).

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Mouse anti RIPK1

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: RIPK1

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VMA00422 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human RIPK1 antibody recognizes the RIPK1, also known as RIP-1, cell death protein RIP, receptor interacting protein, receptor-interacting protein 1, receptor-interacting serine/threonine-protein kinase 1 or serine/threonine-protein kinase RIP.

RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti RIPK1 detects a band of approximately 78 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E.coli-derived recombinant protein corresponding to aa 1-671 of human RIPK1
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q13546
Entrez Gene: RIPK1
GO Terms: GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005739 mitochondrion; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005123 death receptor binding; GO:0005524 ATP binding; GO:0006919 activation of caspase activity; GO:0007257 activation of JUN kinase activity; GO:0008063 Toll signaling pathway; View More GO Terms; GO:0008624 induction of apoptosis by extracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0010008 endosome membrane; GO:0031264 death-inducing signaling complex; GO:0033209 tumor necrosis factor-mediated signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0034612 response to tumor necrosis factor; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043235 receptor complex; GO:0045087 innate immune response; GO:0045768 positive regulation of anti-apoptosis; GO:0046777 protein autophosphorylation; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0060555 induction of necroptosis by extracellular signals; GO:0070513 death domain binding; GO:0070926 regulation of ATP:ADP antiporter activity; GO:0080010 regulation of oxygen and reactive oxygen species metabolic process