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RIPK1 antibody

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Western Blot Validated
Anti RIPK1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with RIPK1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc™ MP with 11 second exposure. Arrow points to RIPK1 (molecular weight 78 kDa)

Mouse anti RIPK1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00422
Datasheet
WB
SDS
100 µl loader

Mouse anti Human RIPK1 antibody recognizes the RIPK1, also known as RIP-1, cell death protein RIP, receptor interacting protein, receptor-interacting protein 1, receptor-interacting serine/threonine-protein kinase 1 or serine/threonine-protein kinase RIP.

RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RIPK1 detects a band of approximately 78 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant protein corresponding to aa 1-671 of human RIPK1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q13546
Entrez Gene
RIPK1
GO Terms
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005123 death receptor binding
GO:0005524 ATP binding
GO:0006919 activation of caspase activity
GO:0007257 activation of JUN kinase activity
GO:0008063 Toll signaling pathway
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0010008 endosome membrane
GO:0031264 death-inducing signaling complex
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0034612 response to tumor necrosis factor
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043235 receptor complex
GO:0045087 innate immune response
GO:0045768 positive regulation of anti-apoptosis
GO:0046777 protein autophosphorylation
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060555 induction of necroptosis by extracellular signals
GO:0070513 death domain binding
GO:0070926 regulation of ATP:ADP antiporter activity
GO:0080010 regulation of oxygen and reactive oxygen species metabolic process
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RIPK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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