RIPK1 antibody

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Mouse anti RIPK1

Mouse anti human RIPK1 antibody recognizes receptor-interacting serine/threonine-protein kinase 1, also known as RIP-1, cell death protein RIP or serine/threonine-protein kinase RIP. This antibody has been extensively validated for western blotting using whole cell lysates.

RIPK1 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00422	WB	100 µl
VMA00422KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human RIPK1 antibody recognizes the RIPK1, also known as RIP-1, cell death protein RIP, receptor interacting protein, receptor-interacting protein 1, receptor-interacting serine/threonine-protein kinase 1 or serine/threonine-protein kinase RIP.

RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: E.coli-derived recombinant protein corresponding to aa 1-671 of human RIPK1

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: Q13546 Related reagents
Entrez Gene: RIPK1 Related reagents
GO Terms: GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005739 mitochondrion; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005123 death receptor binding; GO:0005524 ATP binding; GO:0006919 activation of caspase activity; GO:0007257 activation of JUN kinase activity; GO:0008063 Toll signaling pathway; GO:0008624 induction of apoptosis by extracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0010008 endosome membrane; GO:0031264 death-inducing signaling complex; GO:0033209 tumor necrosis factor-mediated signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0034612 response to tumor necrosis factor; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043235 receptor complex; GO:0045087 innate immune response; GO:0045768 positive regulation of anti-apoptosis; GO:0046777 protein autophosphorylation; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0060555 induction of necroptosis by extracellular signals; GO:0070513 death domain binding; GO:0070926 regulation of ATP:ADP antiporter activity; GO:0080010 regulation of oxygen and reactive oxygen species metabolic process
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of RIPK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti RIPK1 detects a band of approximately 78 kDa in HEK293 cell lysates
Western Blotting: Anti RIPK1 detects a band of approximately 78 kDa in HEK293 cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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