RIPK1 antibody

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Mouse anti RIPK1

Mouse anti human RIPK1 antibody recognizes receptor-interacting serine/threonine-protein kinase 1, also known as RIP-1, cell death protein RIP or serine/threonine-protein kinase RIP. This antibody has been extensively validated for western blotting using whole cell lysates.

RIPK1 Antibody with Control Lysate

Mouse anti human RIPK1 antibody recognizes receptor-interacting serine/threonine-protein kinase 1, also known as RIP-1, cell death protein RIP or serine/threonine-protein kinase RIP. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00421 WB 100 µl
VMA00421KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human RIPK1 antibody recognizes the RIPK1, also known as receptor-interacting protein 1 (RIP1), receptor-interacting serine/threonine-protein kinase 1 or serine/threonine-protein kinase RIP.

RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a bands of 78 and 38 kDa. The full length protein is approximately 78 kDa. The 38 kDa band is believed to be a fragment of the RIPK1 protein. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RIPK1 detects a bands of approximately 78 and 38 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant protein corresponding to aa 1-671 of human RIPK1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q13546 Related reagents
Entrez Gene
RIPK1 Related reagents
GO Terms
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005123 death receptor binding
GO:0005524 ATP binding
GO:0006919 activation of caspase activity
GO:0007257 activation of JUN kinase activity
GO:0008063 Toll signaling pathway
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0010008 endosome membrane
GO:0031264 death-inducing signaling complex
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0034612 response to tumor necrosis factor
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043235 receptor complex
GO:0045087 innate immune response
GO:0045768 positive regulation of anti-apoptosis
GO:0046777 protein autophosphorylation
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060555 induction of necroptosis by extracellular signals
GO:0070513 death domain binding
GO:0070926 regulation of ATP:ADP antiporter activity
GO:0080010 regulation of oxygen and reactive oxygen species metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RIPK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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