RIG-I antibody | OTI6C1

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Anti RIG-I Antibody, clone OTI6C1 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RIG-I Antibody, clone OTI6C1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RIG-I antibody (**VMA00463**) followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 180 second exposure. Arrow points to RIG-I (molecular weight 114 kDa).

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Mouse anti RIG-I

Product Type: PrecisionAb Monoclonal
Clone: OTI6C1
Isotype: IgG2a
Format: Purified
Specificity: RIG-I

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00463 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human RIG-I antibody recognizes RIG-I, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide or retinoic acid-inducible gene 1 protein.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX58 encodes a protein containing RNA helicase-DEAD box protein motifs and two caspase recruitment domains (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. (provided by RefSeq, Jul 2008).

Mouse anti Human RIG-I antibody detects a band of 114 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti RIG-I detects a band of approximately 114 kDa in Jurkat cell lysate.
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant protein of human RIG-I produced in HEK293T cell
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: O95786
Entrez Gene: DDX58
GO Terms: GO:0005515 protein binding; GO:0002230 positive regulation of defense response to virus by host; GO:0003723 RNA binding; GO:0009615 response to virus; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0008026 ATP-dependent helicase activity; GO:0008270 zinc ion binding; GO:0032480 negative regulation of type I interferon production; View More GO Terms; GO:0032727 positive regulation of interferon-alpha production; GO:0032728 positive regulation of interferon-beta production; GO:0045087 innate immune response