RIG-I antibody | 6C1

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Mouse anti RIG-I

Mouse anti-human RIG-1 antibody recognizes the probable ATP-dependent RNA helicase DDX58, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, RNA helicase RIG-I or retinoic acid-inducible gene 1 protein.

RIG-I Antibody with Control Lysate

Mouse anti-human RIG-1 antibody recognizes the probable ATP-dependent RNA helicase DDX58, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, RNA helicase RIG-I or retinoic acid-inducible gene 1 protein.

Product Type
PrecisionAb™ Monoclonal
Clone
6C1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00463 WB 100 µl
VMA00463KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human RIG-I antibody recognizes RIG-I, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide or retinoic acid-inducible gene 1 protein.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX58 encodes a protein containing RNA helicase-DEAD box protein motifs and two caspase recruitment domains (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. (provided by RefSeq, Jul 2008).

Mouse anti Human RIG-I antibody detects a band of 114 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human RIG-I produced in HEK293T cell

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O95786 Related reagents
Entrez Gene
DDX58 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002230 positive regulation of defense response to virus by host
GO:0003723 RNA binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0008270 zinc ion binding
GO:0032480 negative regulation of type I interferon production
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0045087 innate immune response
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RIG-I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti RIG-I detects a band of approximately 114 kDa in Jurkat cell lysate
Western Blotting
Anti RIG-I detects a band of approximately 114 kDa in Jurkat cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB