RIG-I antibody | 6C1

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Mouse anti RIG-I

Product Type
PrecisionAb Monoclonal
Clone
6C1
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00463
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human RIG-I antibody recognizes RIG-I, also known as DEAD box protein 58, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide or retinoic acid-inducible gene 1 protein.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX58 encodes a protein containing RNA helicase-DEAD box protein motifs and two caspase recruitment domains (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. (provided by RefSeq, Jul 2008).

Mouse anti Human RIG-I antibody detects a band of 114 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RIG-I detects a band of approximately 114 kDa in Jurkat cell lysate
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human RIG-I produced in HEK293T cell

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
O95786
Entrez Gene
DDX58
GO Terms
GO:0005515 protein binding
GO:0002230 positive regulation of defense response to virus by host
GO:0003723 RNA binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0008270 zinc ion binding
GO:0032480 negative regulation of type I interferon production
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0045087 innate immune response
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RIG-I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml