RHOA antibody | 21E2-C5

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Mouse anti RHOA

Product Type
PrecisionAb Monoclonal
Clone
21E2-C5
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00872
Datasheet
WB
SDS
100 µl loader

Mouse anti RhoA antibody recognizes transforming protein RhoA, also known as ARH12.

RhoA is a ubiquitously-expressed small GTPase with important roles in cytoskeletal rearrangement, regulation of cell morphology and movement, and transcription. Like other GTPases, it cycles between an inactive GDP-bound state and an active GTP-bound state through exchange of GDP for GTP (Choi et al. 2020). Like many other GTPases, RhoA has been found to play roles in almost all stages of tumor progression including proliferation, migration, invasion and metastasis. Its activation has been shown to contribute to oncogenic transformation of a range of cell types (Dopeso et al. 2018).

Mouse anti RhoA antibody recognizes epitope sequence corresponding to amino acids 171 to 177 of RhoA.

Product Details

Target Species
Human
Western Blotting
Anti RHOA detects a band of approximately 20 kDa
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to RhoA amino acids 159 to 190
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P61586
Entrez Gene
RHOA
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0005856 cytoskeleton
GO:0007266 Rho protein signal transduction
GO:0007411 axon guidance
GO:0017022 myosin binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045666 positive regulation of neuron differentiation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050771 negative regulation of axonogenesis
GO:0050772 positive regulation of axonogenesis
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051496 positive regulation of stress fiber assembly
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RHOA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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