Retinol Binding Protein 4 antibody

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Mouse anti Retinol Binding Protein 4

Retinol binding protein 4 antibody recognizes retinol-binding protein 4, also known as plasma retinol binding protein, PRBP or RBP. The antibody has been extensively validated for western blotting using whole cell lysates.

Retinol Binding Protein 4 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00045	WB	100 µl
VMA00045KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human retinol binding protein 4 antibody recognizes human serum retinol binding protein 4 (RBP4), a 201 amino acid single-chain glycoprotein belonging to the lipocalin family, which is responsible for the transport of Retinol (vitamin A1) from the liver to peripheral target tissues.

In plasma, the RBP4-retinol complex interacts with transthyretin preventing its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency and can cause night vision problems (Besch et al. 2003). RBP4 gene variants have been implicated in insulin resistance (Saucedo et al. 2014)

Mouse anti Human retinol binding protein 4 antibody recognizes free RBP4 and RBP4 in complex with transthyretin. It reacts with apo- as well as holo-RBP4. Mouse anti Human RBP4 antibody recognizes RBP4 as a single band of ~20 kDa in HepG2 cell line whole cell lysates by western blotting under reducing conditions.

Product Details

Target Species: Human
Western Blotting: Anti retinol binding protein 4 antibody detects a band of approximately 20 kDa in HepG2 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Human recombinant retinol binding protein 4
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Guarantee: As supplied, 12 months from date of despatch.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P02753 Related reagents
Entrez Gene: RBP4 Related reagents
GO Terms: GO:0005515 protein binding; GO:0005615 extracellular space; GO:0006094 gluconeogenesis; GO:0016918 retinal binding; GO:0019841 retinol binding; GO:0030277 maintenance of gastrointestinal epithelium; GO:0030324 lung development; GO:0032024 positive regulation of insulin secretion; GO:0032526 response to retinoic acid; GO:0034632 retinol transporter activity; GO:0042572 retinol metabolic process; GO:0042593 glucose homeostasis; GO:0048562 embryonic organ morphogenesis; GO:0048706 embryonic skeletal system development; GO:0048738 cardiac muscle tissue development; GO:0048807 female genitalia morphogenesis; GO:0051024 positive regulation of immunoglobulin secretion; GO:0060044 negative regulation of cardiac muscle cell proliferation; GO:0060059 embryonic retina morphogenesis in camera-type eye; GO:0060065 uterus development; GO:0060068 vagina development; GO:0060157 urinary bladder development; GO:0060347 heart trabecula formation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Retinol Binding Protein 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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