Retinol Binding Protein 4 antibody | RB42

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Mouse anti Human Retinol Binding Protein 4

Product Type: Monoclonal Antibody
Clone: RB42
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA4655	E WB			0.2 mg

Mouse anti Human Retinol Binding Protein 4 antibody, clone RB42 recognizes human serum Retinol Binding Protein 4 (RBP4), a single-chain glycoprotein belonging to the lipocalin family, which is responsible for the transport of Retinol (vitamin A1) from the liver to peripheral target tissues.

In plasma, the RBP4-retinol complex interacts with transthyretin preventing its loss through filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency and can cause night vision problems. RBP4 has been described as an adipokine that contributes to insulin resistance in mice.

Mouse anti Human Retinol Binding Protein 4 antibody, clone RB42 recognizes free RBP4 and RBP4 in complex with transthyretin. It reacts with apo- as well as holo-RBP4. It recognizes human RBP4 in western blotting under reducing conditions.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Human recombinant Retinol Binding Protein 4.
Approx. Protein Concentrations: IgG concentration 1mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P02753 Related reagents
Entrez Gene: RBP4 Related reagents
GO Terms: GO:0005515 protein binding; GO:0005615 extracellular space; GO:0006094 gluconeogenesis; GO:0016918 retinal binding; GO:0019841 retinol binding; GO:0030277 maintenance of gastrointestinal epithelium; GO:0030324 lung development; GO:0032024 positive regulation of insulin secretion; GO:0032526 response to retinoic acid; GO:0034632 retinol transporter activity; GO:0042572 retinol metabolic process; GO:0042593 glucose homeostasis; GO:0048562 embryonic organ morphogenesis; GO:0048706 embryonic skeletal system development; GO:0048738 cardiac muscle tissue development; GO:0048807 female genitalia morphogenesis; GO:0051024 positive regulation of immunoglobulin secretion; GO:0060044 negative regulation of cardiac muscle cell proliferation; GO:0060059 embryonic retina morphogenesis in camera-type eye; GO:0060065 uterus development; GO:0060068 vagina development; GO:0060157 urinary bladder development; GO:0060347 heart trabecula formation
Regulatory: For research purposes only

Applications of Retinol Binding Protein 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Retinol Binding Protein 4 antibody

Reddy, B. M. et al. (1993) Antigenic determinants of human serum retinol binding protein as probed with monoclonal antibodies
Mol Immunol. 30: 1355-60
Sundaram, M. et al. (1998) The transfer of retinol from serum retinol-binding protein to cellular retinol-binding protein is mediated by a membrane receptor
J Biol Chem. 273: 3336-42

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