Retinoblastoma Gene Protein antibody

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Anti Retinoblastoma Gene Protein Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with retinoblastoma gene protein antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to retinoblastoma gene protein (molecular weight 110 kDa).

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Mouse anti Retinoblastoma Gene Protein

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Retinoblastoma Gene Protein

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VMA00668 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human retinoblastoma gene protein antibody recognizes retinoblastoma gene protein, also known as retinoblastoma associated protein, RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).

Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Partial recombinant of human retinoblastoma gene protein
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P06400
Entrez Gene: RB1
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000279 M phase; GO:0000785 chromatin; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003713 transcription coactivator activity; GO:0006338 chromatin remodeling; View More GO Terms; GO:0006469 negative regulation of protein kinase activity; GO:0007050 cell cycle arrest; GO:0007265 Ras protein signal transduction; GO:0008134 transcription factor binding; GO:0016514 SWI/SNF complex; GO:0016564 transcription repressor activity; GO:0016605 PML body; GO:0019900 kinase binding; GO:0030308 negative regulation of cell growth; GO:0030521 androgen receptor signaling pathway; GO:0031575 mitotic cell cycle G1/S transition checkpoint; GO:0031625 ubiquitin protein ligase binding; GO:0035189 Rb-E2F complex; GO:0043433 negative regulation of transcription factor activity; GO:0043550 regulation of lipid kinase activity; GO:0044419 interspecies interaction between organisms; GO:0045445 myoblast differentiation; GO:0045749 negative regulation of S phase of mitotic cell cycle; GO:0050681 androgen receptor binding; GO:0051219 phosphoprotein binding