Retinoblastoma Gene Protein antibody
Mouse anti Retinoblastoma Gene Protein
Retinoblastoma gene protein antibody recognizes retinoblastoma-associated protein, also known as RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.
Retinoblastoma Gene Protein Antibody with Control Lysate
Retinoblastoma gene protein antibody recognizes retinoblastoma-associated protein, also known as RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00668 | WB | 100 µl | ||||
| VMA00668KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human retinoblastoma gene protein antibody recognizes retinoblastoma gene protein, also known as retinoblastoma associated protein, RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.
The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).
Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).
Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Partial recombinant of human retinoblastoma gene protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P06400 Related reagents
- Entrez Gene
- RB1 Related reagents
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000279 M phase
- GO:0000785 chromatin
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0006338 chromatin remodeling
- GO:0006469 negative regulation of protein kinase activity
- GO:0007050 cell cycle arrest
- GO:0007265 Ras protein signal transduction
- GO:0008134 transcription factor binding
- GO:0016514 SWI/SNF complex
- GO:0016564 transcription repressor activity
- GO:0016605 PML body
- GO:0019900 kinase binding
- GO:0030308 negative regulation of cell growth
- GO:0030521 androgen receptor signaling pathway
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0031625 ubiquitin protein ligase binding
- GO:0035189 Rb-E2F complex
- GO:0043433 negative regulation of transcription factor activity
- GO:0043550 regulation of lipid kinase activity
- GO:0044419 interspecies interaction between organisms
- GO:0045445 myoblast differentiation
- GO:0045749 negative regulation of S phase of mitotic cell cycle
- GO:0050681 androgen receptor binding
- GO:0051219 phosphoprotein binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Retinoblastoma Gene Protein antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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