Retinoblastoma Gene Protein antibody

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Mouse anti Retinoblastoma Gene Protein

Retinoblastoma gene protein antibody recognizes retinoblastoma-associated protein, also known as RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

Retinoblastoma Gene Protein Antibody with Control Lysate

Retinoblastoma gene protein antibody recognizes retinoblastoma-associated protein, also known as RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00668 WB 100 µl
VMA00668KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human retinoblastoma gene protein antibody recognizes retinoblastoma gene protein, also known as retinoblastoma associated protein, RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).

Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Partial recombinant of human retinoblastoma gene protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P06400 Related reagents
Entrez Gene
RB1 Related reagents
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000279 M phase
GO:0000785 chromatin
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0006338 chromatin remodeling
GO:0006469 negative regulation of protein kinase activity
GO:0007050 cell cycle arrest
GO:0007265 Ras protein signal transduction
GO:0008134 transcription factor binding
GO:0016514 SWI/SNF complex
GO:0016564 transcription repressor activity
GO:0016605 PML body
GO:0019900 kinase binding
GO:0030308 negative regulation of cell growth
GO:0030521 androgen receptor signaling pathway
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0031625 ubiquitin protein ligase binding
GO:0035189 Rb-E2F complex
GO:0043433 negative regulation of transcription factor activity
GO:0043550 regulation of lipid kinase activity
GO:0044419 interspecies interaction between organisms
GO:0045445 myoblast differentiation
GO:0045749 negative regulation of S phase of mitotic cell cycle
GO:0050681 androgen receptor binding
GO:0051219 phosphoprotein binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Retinoblastoma Gene Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates
Western Blotting
Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB