Retinoblastoma Gene Protein antibody
Mouse anti Human retinoblastoma gene protein antibody recognizes retinoblastoma gene protein, also known as retinoblastoma associated protein, RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.
The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).
Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).
Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Partial recombinant of human retinoblastoma gene protein
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P06400 Related reagents
- Entrez Gene
- RB1 Related reagents
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000279 M phase
- GO:0000785 chromatin
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0006338 chromatin remodeling
- GO:0006469 negative regulation of protein kinase activity
- GO:0007050 cell cycle arrest
- GO:0007265 Ras protein signal transduction
- GO:0008134 transcription factor binding
- GO:0016514 SWI/SNF complex
- GO:0016564 transcription repressor activity
- GO:0016605 PML body
- GO:0019900 kinase binding
- GO:0030308 negative regulation of cell growth
- GO:0030521 androgen receptor signaling pathway
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0031625 ubiquitin protein ligase binding
- GO:0035189 Rb-E2F complex
- GO:0043433 negative regulation of transcription factor activity
- GO:0043550 regulation of lipid kinase activity
- GO:0044419 interspecies interaction between organisms
- GO:0045445 myoblast differentiation
- GO:0045749 negative regulation of S phase of mitotic cell cycle
- GO:0050681 androgen receptor binding
- GO:0051219 phosphoprotein binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Retinoblastoma Gene Protein antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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