Retinoblastoma Gene Protein antibody

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Mouse anti Retinoblastoma Gene Protein

Retinoblastoma gene protein antibody recognizes retinoblastoma-associated protein, also known as RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

Retinoblastoma Gene Protein Antibody with Control Lysate

Retinoblastoma gene protein antibody recognizes retinoblastoma-associated protein, also known as RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00668	WB	100 µl
VMA00668KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human retinoblastoma gene protein antibody recognizes retinoblastoma gene protein, also known as retinoblastoma associated protein, RB transcriptional corepressor 1 and retinoblastoma suspectibility protein.

The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma (provided by RefSeq, Jul 2008).

Mouse anti Human retinoblastoma gene protein antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Partial recombinant of human retinoblastoma gene protein

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P06400 Related reagents
Entrez Gene: RB1 Related reagents
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000279 M phase; GO:0000785 chromatin; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003713 transcription coactivator activity; GO:0006338 chromatin remodeling; GO:0006469 negative regulation of protein kinase activity; GO:0007050 cell cycle arrest; GO:0007265 Ras protein signal transduction; GO:0008134 transcription factor binding; GO:0016514 SWI/SNF complex; GO:0016564 transcription repressor activity; GO:0016605 PML body; GO:0019900 kinase binding; GO:0030308 negative regulation of cell growth; GO:0030521 androgen receptor signaling pathway; GO:0031575 mitotic cell cycle G1/S transition checkpoint; GO:0031625 ubiquitin protein ligase binding; GO:0035189 Rb-E2F complex; GO:0043433 negative regulation of transcription factor activity; GO:0043550 regulation of lipid kinase activity; GO:0044419 interspecies interaction between organisms; GO:0045445 myoblast differentiation; GO:0045749 negative regulation of S phase of mitotic cell cycle; GO:0050681 androgen receptor binding; GO:0051219 phosphoprotein binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of Retinoblastoma Gene Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates
Western Blotting: Mouse anti retinoblastoma gene protein antibody detects a band of approximately 110 kDa in HEK293 cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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