RBM8A antibody | AB02/4G3
Mouse anti RBM8A antibody recognizes RNA-binding protein 8A, also known as ribonucleoprotein RBM8A and Y14.
RBM8A binds to RNA and controls a diverse range of functions including gene expression, cell cycle progression, apoptosis and RNA splicing. This regulation is acheived by employing cancer-related kinases, miRNAs and the E2F1 transcription factor (Lin et al. 2019). Loss of RBM8A leads to increased apoptosis and inhibits cell proliferation. RBM8A is overexpressed in hepatocellular carcinoma (HCC) tissues, and is associated with poor prognosis in patients with HCC (Liang et al. 2017). A role for RBM8A has been suggested in neurodevelopment, and implicated in risk of autism and schizophrenia (Zou et al. 2015). Furthermore, in mice, overexpression of RBM8A led to increased anxiety-like behavior and abnormal social behavior (Alachkar et al. 2013).
RBM8A binds to RNA and controls a diverse range of functions including gene expression, cell cycle progression, apoptosis and RNA splicing. This regulation is acheived by employing cancer-related kinases, miRNAs and the E2F1 transcription factor (Lin et al. 2019). Loss of RBM8A leads to increased apoptosis and inhibits cell proliferation. RBM8A is overexpressed in hepatocellular carcinoma (HCC) tissues, and is associated with poor prognosis in patients with HCC (Liang et al. 2017). A role for RBM8A has been suggested in neurodevelopment, and implicated in risk of autism and schizophrenia (Zou et al. 2015). Furthermore, in mice, overexpression of RBM8A led to increased anxiety-like behavior and abnormal social behavior (Alachkar et al. 2013).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti RBM8A detects a band of approximately 20 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-174 of human RBM8A
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y5S9
- Entrez Gene
- RBM8A
- GO Terms
- GO:0000166 nucleotide binding
- GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003729 mRNA binding
- GO:0005829 cytosol
- GO:0005681 spliceosomal complex
- GO:0006369 termination of RNA polymerase II transcription
- GO:0006406 mRNA export from nucleus
- GO:0006417 regulation of translation
- GO:0016607 nuclear speck
- GO:0031124 mRNA 3'-end processing
- GO:0035145 exon-exon junction complex
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RBM8A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
