RAP1A/B antibody | AB01/2C6.10.4

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Mouse anti RAP1A/B

Product Type
PrecisionAb Monoclonal
Clone
AB01/2C6.10.4
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00546
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human RAP1A/B antibody recognizes the Ras-related protein Rap-1b, also known as GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B or small GTP binding protein.

RAP1B gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of RAP1B are found on chromosomes 3, 5, 6 and 9 (provided by RefSeq, Oct 2011).

Mouse anti Human RAP1A/B antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RAP1A/B detects a band of approximately 21 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human RAP1A/B (amino acids 1 - 184)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P62834 Related reagents
P61224 Related reagents
Entrez Gene
RAP1B Related reagents
RAP1A Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0006112 energy reserve metabolic process
GO:0050796 regulation of insulin secretion
GO:0019003 GDP binding
GO:0000186 activation of MAPKK activity
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of RAP1A/B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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