RAP1A/B antibody | AB01/2C6.10.4
Mouse anti Human RAP1A/B antibody recognizes the Ras-related protein Rap-1b, also known as GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B or small GTP binding protein.
RAP1B gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of RAP1B are found on chromosomes 3, 5, 6 and 9 (provided by RefSeq, Oct 2011).
Mouse anti Human RAP1A/B antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
RAP1B gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of RAP1B are found on chromosomes 3, 5, 6 and 9 (provided by RefSeq, Oct 2011).
Mouse anti Human RAP1A/B antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RAP1A/B detects a band of approximately 21 kDa in K562 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human RAP1A/B (amino acids 1 - 184)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P62834 Related reagents
- P61224 Related reagents
- Entrez Gene
- RAP1B Related reagents
- RAP1A Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0003924 GTPase activity
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005525 GTP binding
- GO:0006112 energy reserve metabolic process
- GO:0050796 regulation of insulin secretion
- GO:0019003 GDP binding
- GO:0000186 activation of MAPKK activity
- GO:0048011 nerve growth factor receptor signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RAP1A/B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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