RALBP1 antibody | OTI11B2
Mouse anti RALBP1
Mouse anti human RALBP1 antibody recognizes RalA-binding protein 1, also known as 76 kDa Ral-interacting protein, RLIP1, RLIP76, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase or RIP1. This antibody has been extensively validated for western blotting using whole cell lysates.
RALBP1 Antibody with Control Lysate
Mouse anti human RALBP1 antibody recognizes RalA-binding protein 1, also known as 76 kDa Ral-interacting protein, RLIP1, RLIP76, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase or RIP1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI11B2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00433 | WB | 100 µl | ||||
| VMA00433KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human RALBP1 antibody recognizes RALBP1, also known as 76 kDa Ral-interacting protein, RLIP1, RLIP76, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase, ral-interacting protein 1 and RIP1.
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020, summary by Feig, 2003, PubMed 12888294, OMIM, Nov 2010).
Mouse anti Human RALBP1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020, summary by Feig, 2003, PubMed 12888294, OMIM, Nov 2010).
Mouse anti Human RALBP1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RALBP1 detects a band of approximately 95 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human RALBP1 (NP_006779) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q15311 Related reagents
- Entrez Gene
- RALBP1 Related reagents
- GO Terms
- GO:0016020 membrane
- GO:0005829 cytosol
- GO:0006810 transport
- GO:0006935 chemotaxis
- GO:0007264 small GTPase mediated signal transduction
- GO:0017160 Ral GTPase binding
- GO:0030675 Rac GTPase activator activity
- GO:0043089 positive regulation of Cdc42 GTPase activity
- GO:0043492 ATPase activity, coupled to movement of substances
- GO:0048365 Rac GTPase binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RALBP1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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