RALBP1 antibody | OTI11B2

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Mouse anti RALBP1

Mouse anti human RALBP1 antibody recognizes RalA-binding protein 1, also known as 76 kDa Ral-interacting protein, RLIP1, RLIP76, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase or RIP1. This antibody has been extensively validated for western blotting using whole cell lysates.

RALBP1 Antibody with Control Lysate

Mouse anti human RALBP1 antibody recognizes RalA-binding protein 1, also known as 76 kDa Ral-interacting protein, RLIP1, RLIP76, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase or RIP1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI11B2
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00433 WB 100 µl
VMA00433KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human RALBP1 antibody recognizes RALBP1, also known as 76 kDa Ral-interacting protein, RLIP1, RLIP76, DNP-SG ATPase, dinitrophenyl S-glutathione ATPase, ral-interacting protein 1 and RIP1.

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020, summary by Feig, 2003, PubMed 12888294, OMIM, Nov 2010).

Mouse anti Human RALBP1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RALBP1 detects a band of approximately 95 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human RALBP1 (NP_006779) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q15311 Related reagents
Entrez Gene
RALBP1 Related reagents
GO Terms
GO:0016020 membrane
GO:0005829 cytosol
GO:0006810 transport
GO:0006935 chemotaxis
GO:0007264 small GTPase mediated signal transduction
GO:0017160 Ral GTPase binding
GO:0030675 Rac GTPase activator activity
GO:0043089 positive regulation of Cdc42 GTPase activity
GO:0043492 ATPase activity, coupled to movement of substances
GO:0048365 Rac GTPase binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RALBP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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