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RAGE antibody | A09-5H8

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Anti Human RAGE Antibody, clone A09-5H8 thumbnail image 1
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Sandwich ELISA analysis of human RAGE using Mouse anti Human RAGE (MCA6276GA) as a capture reagent and biotinylated Mouse anti Human RAGE (MCA6277B) as a detection reagent with purified recombinant RAGE as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

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Mouse anti Human RAGE:Biotin

Product Type
Monoclonal Antibody
Clone
A09-5H8
Isotype
IgG1
Specificity
RAGE

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Product Code Applications Pack Size List Price Your Price Qty
MCA6277B
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E 0.1 mg loader
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Mouse anti Human RAGE antibody, clone A09-5H8, recognizes Receptor for Advanced Glycosylation End products (RAGE), also known as advanced glycosylation end product-specific receptor.

RAGE is a multi-ligand receptor belonging to the immunoglobulin receptor subfamily. It has many endogenous and exogenous ligands and upon binding of a ligand, RAGE can stimulate various signaling pathways including NF-κB, AP-1, CREB and STAT3. Triggering these pathways can then induce cytokine/chemokine transcription (Sorci et al. 2013). This can be used to regulate the innate immune response, inflammation resolution, tissue homeostasis and tissue repair following injury (Plotkin et al. 2019).

The biotinylated Mouse anti Human RAGE antibody, clone A09-5H8 (MCA6277B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human RAGE antibody, clone B02-4G7 (MCA6276GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length RAGE (Ala23-Pro404)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q15109
Entrez Gene
AGER
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0031175 neuron projection development
GO:0045087 innate immune response
GO:0048155 S100 alpha binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity

MCA6277B

100005273

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