RAF-1 (pSer338) antibody | pS338 (DPR/2/2A/3A)

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Rat anti RAF-1 (pSer338)

Product Type
Monoclonal Antibody
Clone
pS338 (DPR/2/2A/3A)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1852 WB* 0.1 mg
Rat anti Raf-1 (pSer338) antibody, clone pS338 (DPR/2/2A/3A) recognizes Raf-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 338.

Rat anti Raf-1 (pSer338) antibody, clone pS338 binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. This antibody does not bind to Raf peptides phosphorylated at other sites, including tyrosine 341 and serine 339.

Rat anti Raf-1 (pSer338) antibody, clone pS338 has been described for the detection of serine 338 phosphorylated Raf-1 by western blotting (Mason et al. 1999).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Synthetic peptide GQRDS*SYY*WEIEAS coupled to glutaraldehyde.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04049 Related reagents
Entrez Gene
RAF1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005057 receptor signaling protein activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007268 synaptic transmission
GO:0007411 axon guidance
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0030168 platelet activation
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of RAF-1 (pSer338) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/100 1/1000
  1. 1For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for RAF-1 (pSer338) antibody

  1. Mason, C.S. et al. (1999) Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.
    EMBO J. 18 (8): 2137-48.
  2. Chiloeches, A. et al. (2001) S338 phosphorylation of Raf-1 is independent of phosphatidylinositol 3-kinase and Pak3.
    Mol Cell Biol. 21: 2423-34.