RAD51 antibody
Mouse anti RAD51
Mouse anti human RAD51 antibody recognizes RAD51 recombinase, also known as RAD51 homolog A, RECA, BRCA1/BRCA2-containing complex, subunit 5 or RAD51A. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00305 | WB | 100 µl |
Mouse anti Human RAD51 antibody recognizes the DNA repair protein RAD51 homolog 1, also known as BRCA1/BRCA2-containing complex, subunit 5, DNA repair protein RAD51 homolog 1, RAD51 homolog A, RecA, E. coli, homolog of, RecA-like protein and recombination protein A.
Encoded by the RAD51 gene, DNA repair protein RAD51 homolog 1 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51 (provided by RefSeq, Aug 2009).
Mouse anti Human DNA repair protein RAD51 homolog 1 antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the RAD51 gene, DNA repair protein RAD51 homolog 1 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51 (provided by RefSeq, Aug 2009).
Mouse anti Human DNA repair protein RAD51 homolog 1 antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti RAD51 detects a band of approximately 37 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombination human RAD51
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q06609 Related reagents
- Entrez Gene
- RAD51 Related reagents
- GO Terms
- GO:0000794 condensed nuclear chromosome
- GO:0000730 DNA recombinase assembly
- GO:0003684 damaged DNA binding
- GO:0003690 double-stranded DNA binding
- GO:0003697 single-stranded DNA binding
- GO:0005524 ATP binding
- GO:0005759 mitochondrial matrix
- GO:0006268 DNA unwinding involved in replication
- GO:0006312 mitotic recombination
- GO:0007131 reciprocal meiotic recombination
- GO:0008022 protein C-terminus binding
- GO:0042802 identical protein binding
- GO:0016605 PML body
- GO:0051260 protein homooligomerization
- GO:0043142 single-stranded DNA-dependent ATPase activity
- GO:0048471 perinuclear region of cytoplasm
- GO:0051106 positive regulation of DNA ligation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of RAD51 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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