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RAD51 antibody

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Anti RAD51 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
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Western blot analysis of whole cell lysates probed with RAD51 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 26 second exposure. Arrow points to RAD51 (molecular weight 37 kDa)

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WB

Mouse anti RAD51

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
RAD51

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VMA00305
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WB 100 µl loader
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Mouse anti Human RAD51 antibody recognizes the DNA repair protein RAD51 homolog 1, also known as BRCA1/BRCA2-containing complex, subunit 5, DNA repair protein RAD51 homolog 1, RAD51 homolog A, RecA, E. coli, homolog of, RecA-like protein and recombination protein A.

Encoded by the RAD51 gene, DNA repair protein RAD51 homolog 1 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51 (provided by RefSeq, Aug 2009).

Mouse anti Human DNA repair protein RAD51 homolog 1 antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RAD51 detects a band of approximately 37 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombination human RAD51
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q06609
Entrez Gene
RAD51
GO Terms
GO:0000794 condensed nuclear chromosome
GO:0000730 DNA recombinase assembly
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0005524 ATP binding
GO:0005759 mitochondrial matrix
GO:0006268 DNA unwinding involved in replication
GO:0006312 mitotic recombination
GO:0007131 reciprocal meiotic recombination
GO:0008022 protein C-terminus binding
GO:0042802 identical protein binding
GO:0016605 PML body
GO:0051260 protein homooligomerization
GO:0043142 single-stranded DNA-dependent ATPase activity
GO:0048471 perinuclear region of cytoplasm
GO:0051106 positive regulation of DNA ligation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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