RAD51 antibody | AB02/1A3

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Anti RAD51 Antibody, clone AB02/1A3 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RAD51 Antibody, clone AB02/1A3 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti RAD51 Antibody, clone AB02/1A3 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RAD51 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to RAD51 (molecular weight 37 kDa)

Anti RAD51 Antibody, clone AB02/1A3 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of RAD51 from Jurkat whole cell lysate. The Input lane is 10% input; the RAD51-IP lane is the immunoprecipitation with the RAD51 antibody (2.5 µg, **VMA00534**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using RAD51 antibody (1/1000, **VMA00534**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5000, 1705047) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to RAD51.

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WB IP

Mouse anti RAD51

Product Type: PrecisionAb Monoclonal
Clone: AB02/1A3
Isotype: IgG1
Format: Purified
Specificity: RAD51

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00534 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human RAD51 antibody recognizes the DNA repair protein RAD51 homolog 1, also known as BRCA1/BRCA2-containing complex,subunit 5, RAD51 homolog A, RecA E. coli or recombination protein A.

The protein encoded by RAD51 gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for RAD51 (provided by RefSeq, Aug 2009).

Mouse anti Human RAD51 antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti RAD51 detects a band of approximately 37 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human RAD51 (amino acids 1 - 339)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q06609
Entrez Gene: RAD51
GO Terms: GO:0000794 condensed nuclear chromosome; GO:0000730 DNA recombinase assembly; GO:0003684 damaged DNA binding; GO:0003690 double-stranded DNA binding; GO:0003697 single-stranded DNA binding; GO:0005524 ATP binding; GO:0005759 mitochondrial matrix; GO:0006268 DNA unwinding involved in replication; GO:0006312 mitotic recombination; View More GO Terms; GO:0007131 reciprocal meiotic recombination; GO:0008022 protein C-terminus binding; GO:0042802 identical protein binding; GO:0016605 PML body; GO:0051260 protein homooligomerization; GO:0043142 single-stranded DNA-dependent ATPase activity; GO:0048471 perinuclear region of cytoplasm; GO:0051106 positive regulation of DNA ligation