RAD50 antibody | AB01/1A7

Mouse anti RAD50

Product Type
PrecisionAb Monoclonal
Clone
AB01/1A7
Isotype
IgG1
Format
Purified
Specificity
RAD50

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VMA00642
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human RAD50 antibody recognizes the DNA repair protein RAD50.

The protein encoded by RAD50 is similar to Saccharomyces cerevisiae. Rad50, a protein involved in DNA double-strand break repair. RAD50 forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities required for non-homologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout of the mouse homolog suggest RAD50 is essential for cell growth and viability. Mutations in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD).

Mouse anti Human RAD50 antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti RAD50 antibody detects a band of approximately 155 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human RAD50 (aa 518-881)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q92878
Entrez Gene
RAD50
GO Terms
GO:0000019 regulation of mitotic recombination
GO:0000724 double-strand break repair via homologous recombination
GO:0000784 nuclear chromosome, telomeric region
GO:0003677 DNA binding
GO:0030674 protein binding, bridging
GO:0004518 nuclease activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0007004 telomere maintenance via telomerase
GO:0007131 reciprocal meiotic recombination
GO:0008270 zinc ion binding
GO:0030870 Mre11 complex
GO:0031954 positive regulation of protein autophosphorylation
GO:0032508 DNA duplex unwinding
GO:0033674 positive regulation of kinase activity
View more products with RAD50 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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