RAD23A antibody | CD01/2B1

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Mouse anti RAD23A

Product Type
PrecisionAb Monoclonal
Clone
CD01/2B1
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00891
Datasheet
WB
SDS
100 µl loader

Mouse anti RAD23A antibody recognizes UV excision repair protein RAD23 homolog A, also known as HR23A.

RAD23A is one of the homologs of yeast Rad23. Both homologs play an important role in DNA repair (Renaud et al. 2011). RAD23A is able to achieve optimal nucleotide excision repair due to the presence of its ubiquitin-binding domain for UBE2D3, which also allows it to interact with the proteasome without destruction (Bethea and Reddy 2015). The yeast protein Rad23 is a ubiquitin shuttle factor, which delivers ubiquitylated proteins to the proteasome (Godderz et al. 2017). RAD23A is a negative regulator of the RIG-I/MDA5-mediated antiviral response (Fang et al. 2013).

Product Details

Target Species
Human
Western Blotting
Mouse anti RAD23A antibody detects a band of approximately 52 kDa in HeLa cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-363 of human RAD23A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P54725
Entrez Gene
RAD23A
GO Terms
GO:0000502 proteasome complex
GO:0003684 damaged DNA binding
GO:0003697 single-stranded DNA binding
GO:0005634 nucleus
GO:0006289 nucleotide-excision repair
GO:0031593 polyubiquitin binding
GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0044419 interspecies interaction between organisms
GO:0045070 positive regulation of viral genome replication
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RAD23A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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