RAD23A antibody | CD01/2B1
Mouse anti RAD23A antibody recognizes UV excision repair protein RAD23 homolog A, also known as HR23A.
RAD23A is one of the homologs of yeast Rad23. Both homologs play an important role in DNA repair (Renaud et al. 2011). RAD23A is able to achieve optimal nucleotide excision repair due to the presence of its ubiquitin-binding domain for UBE2D3, which also allows it to interact with the proteasome without destruction (Bethea and Reddy 2015). The yeast protein Rad23 is a ubiquitin shuttle factor, which delivers ubiquitylated proteins to the proteasome (Godderz et al. 2017). RAD23A is a negative regulator of the RIG-I/MDA5-mediated antiviral response (Fang et al. 2013).
RAD23A is one of the homologs of yeast Rad23. Both homologs play an important role in DNA repair (Renaud et al. 2011). RAD23A is able to achieve optimal nucleotide excision repair due to the presence of its ubiquitin-binding domain for UBE2D3, which also allows it to interact with the proteasome without destruction (Bethea and Reddy 2015). The yeast protein Rad23 is a ubiquitin shuttle factor, which delivers ubiquitylated proteins to the proteasome (Godderz et al. 2017). RAD23A is a negative regulator of the RIG-I/MDA5-mediated antiviral response (Fang et al. 2013).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti RAD23A antibody detects a band of approximately 52 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-363 of human RAD23A
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P54725
- Entrez Gene
- RAD23A
- GO Terms
- GO:0000502 proteasome complex
- GO:0003684 damaged DNA binding
- GO:0003697 single-stranded DNA binding
- GO:0005634 nucleus
- GO:0006289 nucleotide-excision repair
- GO:0031593 polyubiquitin binding
- GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- GO:0044419 interspecies interaction between organisms
- GO:0045070 positive regulation of viral genome replication
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of RAD23A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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