RAB5A antibody | rCD01-2G10

100% Secure


Mouse anti RAB5A

Product Type
PrecisionAb Monoclonal
Clone
rCD01-2G10
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00902
Datasheet
WB
SDS
100 µl loader

Mouse anti RAB5A antibody recognizes ras-related protein Rab-5A, also known as RAB5.

RAB5A is a member of the Rab family of small GTPases and acts as an important rate-limiting component for regulation of endocytosis and early endosome fusion, and also plays a role in autophagy (Xu et al. 2018). Accordingly, RAB5A regulates transport between the plasma membrane and early endosomes (Bucci et al. 1994). However, RAB5A plays diverse biological roles. In oocytes during meiosis, the protein is required for control of spindle length and kinetochore-microtubule attachment (Ma et al. 2014). RAB5A is also involved with regulation of T cell motility, important for the adaptive immune response (Ong et al. 2014). Rab proteins play a role in the regulation of cancer progression, and RAB5A is overexpressed in several human cancers (Zhang et al. 2017). More specifically, aberrant RAB5A expression contributes to increased distant metastasis. For example, RAB5A is involved with metastatic potential of lung and gastric cancer (Yu et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti RAB5A antibody detects a band of approximately 25 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Recombinant IgG prepared by affinity chromatography on Protein A from cell culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli--derived recombinant protein of amino acids 1-215 of human RAB5A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P20339
Entrez Gene
RAB5A
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0007596 blood coagulation
GO:0005525 GTP binding
GO:0007264 small GTPase mediated signal transduction
GO:0015031 protein transport
GO:0019003 GDP binding
GO:0031623 receptor internalization
GO:0031901 early endosome membrane
GO:0042470 melanosome
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RAB5A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra