RAB5A antibody | rCD01-2G10

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Anti RAB5A Antibody, clone rCD01-2G10 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti RAB5A Antibody, clone rCD01-2G10 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti RAB5A Antibody, clone rCD01-2G10 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti RAB5A Antibody, clone rCD01-2G10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with RAB5A antibody (1/1,000, **VMA00902**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to RAB5A (molecular weight 25 kDa).

Anti RAB5A Antibody, clone rCD01-2G10 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against RAB5A (1 & 2) or a scrambled siRNA (-) probed with Mouse anti RAB5A antibody (**VMA00902**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 189 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti RAB5A Antibody, clone rCD01-2G10 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of RAB5A from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against RAB5A (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti RAB5A

Product Type: PrecisionAb Monoclonal
Clone: rCD01-2G10
Isotype: IgG2a
Format: Purified
Specificity: RAB5A

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VMA00902 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti RAB5A antibody recognizes ras-related protein Rab-5A, also known as RAB5.

RAB5A is a member of the Rab family of small GTPases and acts as an important rate-limiting component for regulation of endocytosis and early endosome fusion, and also plays a role in autophagy (Xu et al. 2018). Accordingly, RAB5A regulates transport between the plasma membrane and early endosomes (Bucci et al. 1994). However, RAB5A plays diverse biological roles. In oocytes during meiosis, the protein is required for control of spindle length and kinetochore-microtubule attachment (Ma et al. 2014). RAB5A is also involved with regulation of T cell motility, important for the adaptive immune response (Ong et al. 2014). Rab proteins play a role in the regulation of cancer progression, and RAB5A is overexpressed in several human cancers (Zhang et al. 2017). More specifically, aberrant RAB5A expression contributes to increased distant metastasis. For example, RAB5A is involved with metastatic potential of lung and gastric cancer (Yu et al. 2015).

Target Species: Human
Western Blotting: Mouse anti RAB5A antibody detects a band of approximately 25 kDa in HeLa cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Recombinant IgG prepared by affinity chromatography on Protein A from cell culture supernatant
Source: CHO-S
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: E. coli--derived recombinant protein of amino acids 1-215 of human RAB5A
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: As supplied, 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P20339
Entrez Gene: RAB5A
GO Terms: GO:0005886 plasma membrane; GO:0003924 GTPase activity; GO:0007596 blood coagulation; GO:0005525 GTP binding; GO:0007264 small GTPase mediated signal transduction; GO:0015031 protein transport; GO:0019003 GDP binding; GO:0031623 receptor internalization; GO:0031901 early endosome membrane; View More GO Terms; GO:0042470 melanosome