RAB1A antibody | EF02/1D7

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Mouse anti RAB1A

Product Type
PrecisionAb Monoclonal
Clone
EF02/1D7
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00959
Datasheet
WB
SDS
100 µl loader

Mouse anti RAB1A antibody recognizes ras-related protein Rab-1A, also known as YPT1-related protein. RAB1A is a member of the RAB family of GTPases, and regulates traffic of vesicles from the endoplasmic reticulum to the Golgi apparatus. RAB1A also functions in mediation of signal transduction, cell migration, and autophagy regulation (Cheng et al. 2019). RAB1A plays an oncogenic role in many human cancers including breast cancer, lung cancer, hepatocellular carcinoma (Wang et al. 2018) and colorectal cancer (Thomas et al. 2014). Importantly for various cancer types, RAB1A is involved with regulating the PI3K-AKT-mTORC1 pathway through the mTORC1 complex (Xu et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti RAB1A detects a band of approximately 23 kDa in MCF-7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-205 of human RAB1A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P62820
Entrez Gene
RAB1A
GO Terms
GO:0003924 GTPase activity
GO:0016192 vesicle-mediated transport
GO:0005783 endoplasmic reticulum
GO:0005525 GTP binding
GO:0005794 Golgi apparatus
GO:0007264 small GTPase mediated signal transduction
GO:0015031 protein transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RAB1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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