Pyruvate Kinase PKLR antibody | OTI1F3

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Mouse anti Pyruvate Kinase PKLR

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human pyruvate kinase PKLR antibody, clone OTI1F3 recognizes the pyruvate kinase PKLR, also known as R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase PKLR, pyruvate kinase isozyme R/L, pyruvate kinase isozymes L/R, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and blood cell and red cell/liver pyruvate kinase.

Encoded by PKLR gene, pyruvate kinase PKLR catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for PKLR (provided by RefSeq, Jul 2008).

Mouse anti Human pyruvate kinase PKLR antibody, clone OTI1F3 detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Anti pyruvate kinase PKLR detects a band of approximately 62 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Full length recombinant human pyruvate kinase PKLR (NP_000289) produced in HEK293T cells

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0000287 magnesium ion binding
GO:0005829 cytosol
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0006096 glycolysis
GO:0006112 energy reserve metabolic process
GO:0030955 potassium ion binding
GO:0031018 endocrine pancreas development
GO:0031325 positive regulation of cellular metabolic process
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of Pyruvate Kinase PKLR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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