Pyruvate Kinase PKLR antibody | OTI1F3

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Mouse anti Pyruvate Kinase PKLR

Pyruvate kinase PKLR antibody recognizes pyruvate kinase PKLR, also known as R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase isozyme R/L, or red cell/liver pyruvate kinase. This antibody has been extensively validated for western blotting using whole cell lysates.

Pyruvate Kinase PKLR Antibody with Control Lysate

Pyruvate kinase PKLR antibody recognizes pyruvate kinase PKLR, also known as R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase isozyme R/L, or red cell/liver pyruvate kinase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1F3
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00264 WB 100 µl
VMA00264KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human pyruvate kinase PKLR antibody recognizes the pyruvate kinase PKLR, also known as R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase PKLR, pyruvate kinase isozyme R/L, pyruvate kinase isozymes L/R, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and blood cell and red cell/liver pyruvate kinase.

Encoded by PKLR gene, pyruvate kinase PKLR catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for PKLR (provided by RefSeq, Jul 2008).

Mouse anti Human pyruvate kinase PKLR antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human pyruvate kinase PKLR (NP_000289) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P30613 Related reagents
Entrez Gene
PKLR Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0005829 cytosol
GO:0004743 pyruvate kinase activity
GO:0005524 ATP binding
GO:0006096 glycolysis
GO:0006112 energy reserve metabolic process
GO:0030955 potassium ion binding
GO:0031018 endocrine pancreas development
GO:0031325 positive regulation of cellular metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Pyruvate Kinase PKLR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti pyruvate kinase PKLR detects a band of approximately 62 kDa in K562 cell lysates
Western Blotting
Anti pyruvate kinase PKLR detects a band of approximately 62 kDa in K562 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB