PTPN11/6 antibody

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Mouse anti PTPN11/6

PTPN11/6 antibody recognizes the tyrosine-protein phosphatase non-receptor type 11, also known as PTP-2C, protein-tyrosine phosphatase 1D, or protein-tyrosine phosphatase 2C. This antibody has been extensively validated for western blotting using whole cell lysates.

PTPN11/6 Antibody with Control Lysate

PTPN11/6 antibody recognizes the tyrosine-protein phosphatase non-receptor type 11, also known as PTP-2C, protein-tyrosine phosphatase 1D, or protein-tyrosine phosphatase 2C. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00090 WB 100 µl
VMA00090KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PTPN11/6 antibody recognizes the tyrosine-protein phosphatase non-receptor type 11, also known as PTP-2C, protein-tyrosine phosphatase 1D, protein-tyrosine phosphatase 2C and tyrosine-protein phosphatase non-receptor type 11. This antibody has also been shown to recognize the tyrosine-protein phosphatase non-receptor type 6, also known as HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L and SHP1.

The proteins encoded by PTPN11 and the PTPN6 genes are members of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Mouse anti Human PTPN11/6 antibody detects a band of 68 kDa in whole cell lysates. The antibody has been extensively validated for western blotting.

Product Details

Target Species
Human
Western Blotting
Anti PTPN11/6 detects a band of approximately 68 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein of human PTPN11
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q06124 Related reagents
Entrez Gene
PTPN11 Related reagents
GO Terms
GO:0005829 cytosol
GO:0004726 non-membrane spanning protein tyrosine phosphatase activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007411 axon guidance
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0034329 cell junction assembly
GO:0050900 leukocyte migration
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PTPN11/6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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