PSMD10 antibody | AB03/4A8
Mouse anti PSMD10 antibody recognizes 26S proteasome non-ATPase regulatory subunit 10, also known as gankyrin, p28(GANK).
PSMD10 is one of the nonATPase subunits of the regulatory complex of the 26S proteasome. PSMD10 facilitates degradation of RB1 and TP53, controlling the balance between cell cycle progression and apoptosis (Luo et al. 2016). It functions as an oncoprotein in the liver, is overexpressed in hepatocellular carcinoma (HCC), and is overexpressed in prostate tumors (Kim et al. 2017). In HCC, PSMD10 promotes autophagy in response to environmental stressors to increase cancer progression (Luo et al. 2016). Dysregulation of PSMD10 promotes tumor progression, cell proliferation and metastasis. PSMD10 has been identified as a potential therapeutic target for certain cancer types (Liu et al. 2018)
PSMD10 is one of the nonATPase subunits of the regulatory complex of the 26S proteasome. PSMD10 facilitates degradation of RB1 and TP53, controlling the balance between cell cycle progression and apoptosis (Luo et al. 2016). It functions as an oncoprotein in the liver, is overexpressed in hepatocellular carcinoma (HCC), and is overexpressed in prostate tumors (Kim et al. 2017). In HCC, PSMD10 promotes autophagy in response to environmental stressors to increase cancer progression (Luo et al. 2016). Dysregulation of PSMD10 promotes tumor progression, cell proliferation and metastasis. PSMD10 has been identified as a potential therapeutic target for certain cancer types (Liu et al. 2018)
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti PSMD10 antibody detects a band of approximately 23 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-226 of human PSMD10
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O75832
- Entrez Gene
- PSMD10
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0006915 apoptosis
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005838 proteasome regulatory particle
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0007253 cytoplasmic sequestering of NF-kappaB
- GO:0008134 transcription factor binding
- GO:0016032 viral reproduction
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0015629 actin cytoskeleton
- GO:0016071 mRNA metabolic process
- GO:0030307 positive regulation of cell growth
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0043066 negative regulation of apoptosis
- GO:0043409 negative regulation of MAPKKK cascade
- GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
- GO:0045111 intermediate filament cytoskeleton
- GO:0045737 positive regulation of cyclin-dependent protein kinase activity
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0070682 proteasome regulatory particle assembly
- GO:0090201 negative regulation of release of cytochrome c from mitochondria
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PSMD10 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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