PSMD10 antibody | AB03/4A8

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Mouse anti PSMD10

Product Type
PrecisionAb Monoclonal
Clone
AB03/4A8
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00859
Datasheet
WB
SDS
100 µl loader

Mouse anti PSMD10 antibody recognizes 26S proteasome non-ATPase regulatory subunit 10, also known as gankyrin, p28(GANK).

PSMD10 is one of the nonATPase subunits of the regulatory complex of the 26S proteasome. PSMD10 facilitates degradation of RB1 and TP53, controlling the balance between cell cycle progression and apoptosis (Luo et al. 2016). It functions as an oncoprotein in the liver, is overexpressed in hepatocellular carcinoma (HCC), and is overexpressed in prostate tumors (Kim et al. 2017). In HCC, PSMD10 promotes autophagy in response to environmental stressors to increase cancer progression (Luo et al. 2016). Dysregulation of PSMD10 promotes tumor progression, cell proliferation and metastasis. PSMD10 has been identified as a potential therapeutic target for certain cancer types (Liu et al. 2018)

Product Details

Target Species
Human
Western Blotting
Mouse anti PSMD10 antibody detects a band of approximately 23 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-226 of human PSMD10
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O75832
Entrez Gene
PSMD10
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005838 proteasome regulatory particle
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0008134 transcription factor binding
GO:0016032 viral reproduction
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015629 actin cytoskeleton
GO:0016071 mRNA metabolic process
GO:0030307 positive regulation of cell growth
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0043066 negative regulation of apoptosis
GO:0043409 negative regulation of MAPKKK cascade
GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045111 intermediate filament cytoskeleton
GO:0045737 positive regulation of cyclin-dependent protein kinase activity
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0070682 proteasome regulatory particle assembly
GO:0090201 negative regulation of release of cytochrome c from mitochondria
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PSMD10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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