The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMC5 gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for PSMC5 (provided by RefSeq, Nov 2010).
Mouse anti Human PSMC5 detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti PSMC5 detects a band of approximately 46 kDa in HEK293 cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant protein corresponding to amino acids 1-130 of human PSMC5 expressed in E. coli
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0000502 proteasome complex
- GO:0003712 transcription cofactor activity
- GO:0006915 apoptosis
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0008134 transcription factor binding
- GO:0016887 ATPase activity
- GO:0016032 viral reproduction
- GO:0016071 mRNA metabolic process
- GO:0042981 regulation of apoptosis
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0031531 thyrotropin-releasing hormone receptor binding
- GO:0043069 negative regulation of programmed cell death
- GO:0043193 positive regulation of gene-specific transcription
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of PSMC5 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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