PSMB9 antibody | OTI2B6

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Anti PSMB9 Antibody, clone OTI2B6 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti PSMB9 Antibody, clone OTI2B6 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PSMB9 antibody (**VMA00470**) followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to PSMB9 (molecular weight 23 kDa).

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Mouse anti PSMB9

Product Type: PrecisionAb Monoclonal
Clone: OTI2B6
Isotype: IgG1
Format: Purified
Specificity: PSMB9

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00470 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human PSMB9 antibody recognizes the proteasome subunit beta type-9, also known as large multifunctional peptidase 2, low molecular mass protein 2, macropain chain 7, multicatalytic endopeptidase complex chain 7, proteasome chain 7, or proteasome subunit beta-1i.

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB9 gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. PSMB9 is located in the class II region of the MHC (major histocompatibility complex). Expression of PSMB9 is induced by gamma interferon and PSMB9 product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit (provided by RefSeq, Mar 2010).

Mouse anti Human PSMB9 antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PSMB9 detects a band of approximately 23 kDa in HEK293 cell lysate.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9 produced in E. Coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P28065
Entrez Gene: PSMB9
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0006915 apoptosis; GO:0004298 threonine-type endopeptidase activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005839 proteasome core complex; GO:0006521 regulation of cellular amino acid metabolic process; View More GO Terms; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0016032 viral reproduction; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0044419 interspecies interaction between organisms; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle