PSMB2 antibody | GH01/3E1

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Mouse anti PSMB2

Product Type
PrecisionAb Monoclonal
Clone
GH01/3E1
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00954
Datasheet
WB
SDS
100 µl loader

Mouse anti PSMB2 antibody recognizes proteasome subunit beta type-2, also known as macropain subunit C7-I. PSMB2 is a component of the 20S proteasome, a large protein complex which has critical roles in regulating homeostasis by degrading targeted proteins (Latham et al. 2014). Proteins become tagged for destruction when they acquire a polymerized ubiquitin chain, shutting the protein to the proteasome. In this way, the proteasome is able to selectively degrade specific proteins. Through its ability to degrade a diverse range of tagged proteins, the proteasome plays a wide variety of roles in biological processes by rapid catalysis of biological reactions (Tanaka et al. 2009).

Product Details

Target Species
Human
Western Blotting
Mouse anti PSMB2 detects a band of approximately 24 kDa in K562 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-201 of human PSMB2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P49721
Entrez Gene
PSMB2
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0006915 apoptosis
GO:0004298 threonine-type endopeptidase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005839 proteasome core complex
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0016032 viral reproduction
GO:0016071 mRNA metabolic process
GO:0042981 regulation of apoptosis
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0044419 interspecies interaction between organisms
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PSMB2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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