PSMB10 antibody | AB01/4E9
Mouse anti PSMB10 antibody recognizes proteasome subunit beta type-10, also known as LMP10 and MECL1.
PSMB10 is a catalytic subunit of the 26S proteasome complex, which is important for protein degradation and for generation of peptides that bind to MHC molecules (Wu et al. 2006). PSMB10 forms part of the antigen-presenting machinery, and is regulated by interferon gamma (Ayers et al. 2017). The importance of PSMB10 in cardiovascular disease is being dissected, with roles of PSMB10 suggested in hypertensive retinopathy (Wang et al. 2018), hypertensive atrial fibrillation (Li et al. 2018) and abdominal aortic aneurysm (Butt et al. 2016).
PSMB10 is a catalytic subunit of the 26S proteasome complex, which is important for protein degradation and for generation of peptides that bind to MHC molecules (Wu et al. 2006). PSMB10 forms part of the antigen-presenting machinery, and is regulated by interferon gamma (Ayers et al. 2017). The importance of PSMB10 in cardiovascular disease is being dissected, with roles of PSMB10 suggested in hypertensive retinopathy (Wang et al. 2018), hypertensive atrial fibrillation (Li et al. 2018) and abdominal aortic aneurysm (Butt et al. 2016).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti PSMB10 detects a band of approximately 26 kDa in Raji cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 40-273 of human PSMB10
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P40306
- Entrez Gene
- PSMB10
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0006915 apoptosis
- GO:0004298 threonine-type endopeptidase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005839 proteasome core complex
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006959 humoral immune response
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0016032 viral reproduction
- GO:0016071 mRNA metabolic process
- GO:0042981 regulation of apoptosis
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0044419 interspecies interaction between organisms
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PSMB10 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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