PSMA1 antibody | CD01/1C10
Mouse anti PSMA1 antibody recognizes proteasome subunit alpha type-1, also known as HC2, PROS30, PSC2. PSMA1 is one of the 17 essential subunits of the 20S proteasome, a complex which has critical roles in regulating homeostasis by degrading targeted proteins (Latham et al. 2014). The proteasome pathway is closely linked to cell cycle progression and apoptosis, providing a link to cancer. Expression of PSMA1 has been found to be reduced in tissue interstitial fluid in hepatocellular carcinoma compared with normal interstitial fluid of adjacent nontumor tissues (Qin et al. 2017). However, PSMA1 overexpression has been identified in colorectal cancer and breast cancer (Yang et al. 2017).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti PSMA1 detects a band of approximately 29 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-263 of human PSMA1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P25786
- Entrez Gene
- PSMA1
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0003723 RNA binding
- GO:0006915 apoptosis
- GO:0004298 threonine-type endopeptidase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005844 polysome
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0016032 viral reproduction
- GO:0016071 mRNA metabolic process
- GO:0042981 regulation of apoptosis
- GO:0019773 proteasome core complex, alpha-subunit complex
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PSMA1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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