PSIP1 antibody | AB01/1A2
Mouse anti PSIP1
PSIP1 antibody recognizes PSIP1, also known as PC4 and SFRS1-interacting protein, CLL-associated antigen KW-7, DFS 70 or transcriptional coactivator p52/p75.
PSIP1 Antibody with Control Lysate
PSIP1 antibody recognizes PSIP1, also known as PC4 and SFRS1-interacting protein, CLL-associated antigen KW-7, DFS 70 or transcriptional coactivator p52/p75.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB01/1A2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00638 | WB | 100 µl | ||||
| VMA00638KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human PSIP1 antibody recognizes PSIP1, also known as PC4 and SFRS1-interacting protein, CLL-associated antigen KW-7, DFS 70 or transcriptional coactivator p52/p75.
Mouse anti Human PSIP1 antibody detects a bands or 76, 51 and 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human PSIP1 antibody detects a bands or 76, 51 and 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti PSIP1 antibody detects bands of approximately 76, 51 & 45 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human PSIP1 (aa 1-324)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O75475 Related reagents
- Entrez Gene
- PSIP1 Related reagents
- GO Terms
- GO:0003677 DNA binding
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0006350 transcription
- GO:0019047 provirus integration
- GO:0019059 initiation of viral infection
- GO:0044419 interspecies interaction between organisms
- GO:0045449 regulation of transcription
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PSIP1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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