PRPF8 antibody | AB01/4B2
Mouse anti PRPF8
PRPF8 antibody recognizes pre-mRNA-processing-splicing factor 8, also known as 220 kDa U5 snRNP-specific protein, apoptosis-regulated protein 1 or precursor mRNA processing protein.
PRPF8 Antibody with Control Lysate
PRPF8 antibody recognizes pre-mRNA-processing-splicing factor 8, also known as 220 kDa U5 snRNP-specific protein, apoptosis-regulated protein 1 or precursor mRNA processing protein.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- AB01/4B2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00648 | WB | 100 µl | ||||
| VMA00648KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human PRPF8 antibody recognizes pre-mRNA-processing-splicing factor 8, also known as 220 kDa U5 snRNP-specific protein, apoptosis-regulated protein 1 or precursor mRNA processing protein.
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by PRPF8 is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. PRPF8 is a candidate gene for autosomal dominant retinitis pigmentosa (provided by RefSeq, Jul 2008).
Mouse anti Human PRPF8 antibody detects a band of 252 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by PRPF8 is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. PRPF8 is a candidate gene for autosomal dominant retinitis pigmentosa (provided by RefSeq, Jul 2008).
Mouse anti Human PRPF8 antibody detects a band of 252 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti PRPF8 antibody detects a band of approximately 252 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli-derived recombinant human PRPF8 (aa 1-467)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q6P2Q9 Related reagents
- Entrez Gene
- PRPF8 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003723 RNA binding
- GO:0005681 spliceosomal complex
- GO:0005682 U5 snRNP
- GO:0007601 visual perception
- GO:0016607 nuclear speck
- GO:0050896 response to stimulus
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PRPF8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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