Protein Kinase C zeta antibody | AB02/3G7
Protein kinase C zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).
Mouse anti Human protein kinase C zeta antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti protein kinase C zeta detects a band of approximately 78 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- E. coli-derived recombinant human protein kinase C zeta (amino acids 454 - 592)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- Q05513 Related reagents
- Entrez Gene
- PRKCZ Related reagents
- GO Terms
- GO:0004697 protein kinase C activity
- GO:0005524 ATP binding
- GO:0005768 endosome
- GO:0006916 anti-apoptosis
- GO:0008270 zinc ion binding
- GO:0018105 peptidyl-serine phosphorylation
- GO:0030168 platelet activation
- GO:0031333 negative regulation of protein complex assembly
- GO:0043560 insulin receptor substrate binding
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of Protein Kinase C zeta antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||2 ml||WB|
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