Protein Kinase C Delta antibody

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Mouse anti Protein Kinase C Delta

Mouse anti human protein kinase C delta antibody recognizes tyrosine protein kinase PRKCD, also known as NPKC-delta or protein kinase C delta VIII. This antibody has been extensively validated for western blotting using whole cell lysates.

Protein Kinase C Delta Antibody with Control Lysate

Mouse anti human protein kinase C delta antibody recognizes tyrosine protein kinase PRKCD, also known as NPKC-delta or protein kinase C delta VIII. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00408 WB 100 µl
VMA00408KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human protein kinase C delta antibody recognizes protein kinase C delta also known as tyrosine-protein kinase PRKCD.

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by PRKCD is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed (provided by RefSeq, Jul 2008).

Mouse anti Human protein kinase C delta antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti protein kinase C delta detects a band of approximately 78 kDa in A431 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant protein corresponding to aa 549-676 of human protein kinase C delta
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A431 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q05655 Related reagents
Entrez Gene
PRKCD Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004699 calcium-independent protein kinase C activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007202 activation of phospholipase C activity
GO:0008022 protein C-terminus binding
GO:0008047 enzyme activator activity
GO:0010469 regulation of receptor activity
GO:0016071 mRNA metabolic process
GO:0050821 protein stabilization
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0032091 negative regulation of protein binding
GO:0043560 insulin receptor substrate binding
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0090398 cellular senescence
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Protein Kinase C Delta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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